Microfluidic Devices and Methods of Using Same
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Abstract
An M.times.N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.
211 Citations
16 Claims
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1-12. -12. (canceled)
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13. A microfluidic device for reacting a plurality of different samples with a plurality of different reagents comprising:
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a) a first layer; b) a second layer; c) a plurality of reaction cells located in said second layer; wherein each reaction cell comprises; i) a first chamber located in the second layer; ii) a second chamber located in the second layer connected to the first chamber via an interface channel; iii) a first valve in said interface channel between said first chamber and said second chamber that, when actuated, fluidically isolates said first chamber and said second chamber from each other; wherein each said first chamber is in fluid communication with a first fluid channel located in said second layer, each said first fluid channel is in fluid communication with a plurality of first chambers, and each said first fluid channel is in fluid communication with a fluid inlet through which solution can be introduced into said channel; wherein each said second chamber is in fluid communication with a second fluid channel that traverses an interface between said first and second layers; and
wherein a plurality of said second fluid channels are in fluid communication with a single third fluid channel, and each said third fluid channel is located in said first layer and is in fluidic communication with a fluid inlet through which solution can be introduced into said channel. - View Dependent Claims (14, 15, 16)
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Specification