Albumin Denaturing Agent

• US 20090142787A1
• Filed: 10/27/2006
• Published: 06/04/2009
• Est. Priority Date: 10/27/2005
• Status: Active Grant

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Norio, Inamura, Yonehara, Satoshi
• Granted Patent: US 7,871,789 B2
• Time in Patent Office: Days
• US Class Current: 435/23
• CPC Class Codes: C12Q 1/37 involving peptidase or proteinase
• CPC Class Codes: G01N 2333/76 Assays involving albumins other than in routine use for blocking surfaces or for anchoring haptens during immunisation
• CPC Class Codes: G01N 2400/00 Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
• CPC Class Codes: G01N 33/68 involving proteins, peptides or amino acids involving lipoproteins G01N33/92

Abstract

An albumin denaturing agent for digesting an albumin by a protease efficiently is provided. The albumin denaturing agent contains quaternary ammonium having a hydrocarbon group with a carbon number of 12 or more, or a salt of the quaternary ammonium. The albumin in a sample is digested by the protease in the presence of the albumin denaturing agent, a glycated part of the thus obtained albumin digestion product and a FAOD effect a reaction, and a redox reaction between the glycated part and the FAOD is measured, thereby determining a ratio (GA (%)) of the glycated albumin of the glycated albumin with respect to the albumin.

Claims

1. An albumin denaturing composition comprising quaternary ammonium having a hydrocarbon group with a carbon number of 12 or more, or a salt of the quaternary ammonium and protease.

2. The albumin denaturing composition according to claim 1, comprising at least one quaternary ammonium selected from the group consisting of:

stearyltrimethylammonium chloride;

cetyltrimethylammonium bromide;

hexadecyltrimethylammonium bromide;

benzalkonium chloride;

benzyldimethyltetradecylammonium chloride;

benzethonium chloride;

myristyltrimetylammonium bromide;

coconut amine acetate; and

lauryltrimethylammonium chloride, or a salt of the quaternary ammonium.

3. The albumin denaturing composition according to claim 1, comprising quaternary ammonium having a hydrocarbon group with a carbon number of 14 or more, or a salt of the quaternary ammonium.

4. The albumin denaturing composition according to claim 1, comprising a mixture of quaternary ammonium having a hydrocarbon group with a carbon number of 14 or more, or salts of the quaternary ammonium.

5. The albumin denaturing composition according to claim 1, comprising quaternary ammonium having a hydrocarbon group with a carbon number of 16 or more, or a salt of the quaternary ammonium.

6. The albumin denaturing composition according to claim 1, comprising quaternary ammonium having a hydrocarbon group with a carbon number of 14 to 18, or salts of the quaternary ammonium.

7. The albumin denaturing composition according to claim 3, comprising at least one quaternary ammonium selected from the group consisting of:

stearyltrimethylammonium chloride;

cetyltrimethylammonium bromide;

hexadecyltrimethylammonium bromide;

benzalkonium chloride;

benzyldimethyltetradecylammonium chloride;

benzethonium chloride;

myristyltrimetylammonium bromide; and

coconut amine acetate, or a salt of the quaternary ammonium.

8. The albumin denaturing composition according to claim 1, wherein 1 U to 1,600,000 U of the protease is present per 0.02 mmol to 20 mmol of the quaternary ammonium.

9. The albumin denaturing composition according to claim 1, wherein 1 U to 500,000 U of the protease is present per 0.02 mmol to 20 mmol of the quaternary ammonium.

10. The albumin denaturing composition according to claim 1, which is used by adding 0.003 mmol to 4 mmol of the quaternary ammonium and 1 U to 60,000 U of the protease into 1 ml of a sample.

11. The albumin denaturing composition according to claim 1, which is used by adding 0.003 mmol to 4 mmol of the quaternary ammonium and 1 U to 35,000 U of the protease into 1 ml of a sample.

12-13. -13. (canceled)

14. An albumin digesting method for digesting an albumin, comprising:

a step of adding a protease into a sample containing the albumin; and

a step of adding quaternary ammonium having a hydrocarbon group with a carbon number of 12 or more, or a salt of the quaternary ammonium before the adding of the protease, at the same time of the adding or after the adding, wherein a resultant concentration of the protease ranges from 1 U/L to 1,600,000 U/L.

15. The albumin digesting method according to claim 14, wherein the resultant concentration of the protease ranges from 1 U/L to 500,000 U/L.

16. An albumin digesting method for digesting an albumin, comprising:

a step of adding a protease into a sample containing the albumin; and

a step of adding quaternary ammonium having a hydrocarbon group with a carbon number of 12 or more, or a salt of the quaternary ammonium before the adding of the protease, at the same time of the adding or after the adding, wherein an adding amount of the protease ranges from 1 U to 60,000 U in 1 ml of the sample.

17. The albumin digesting method according to claim 16, wherein the adding amount of the protease ranges from 1 U to 35,000 U in 1 ml of the sample.

18. An method for assaying glycated albumin comprising:

a step of adding a protease into a sample containing the albumin; and

a step of adding quaternary ammonium having a hydrocarbon group with a carbon number of 12 or more, or a salt of the quaternary ammonium before, at the same time of or after the adding of the protease, wherein a resultant concentration of the protease ranges from 1 U/L to 1,600,000 U/L.

19. An method for assaying glycated albumin comprising:

a step of adding a protease into a sample containing the albumin; and

a step of adding quaternary ammonium having a hydrocarbon group with a carbon number of 12 or more, or a salt of the quaternary ammonium before, at the same time of or after the adding of the protease, wherein an adding amount of the protease ranges from 1 U to 60,000 U in 1 ml of the sample.