METHOD FOR SYNTHESISING TEMPLATED MOLECULES
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Abstract
The invention relates to a method for synthesizing templated molecules attached to the templated which directed the synthesis thereof. The method involves a template, a scaffold functional entity and a functional entity attached to a building block, which, in turn, is attached the template. The scaffold functional entity and the functional entity of the building block are both provided with complementary dimerization domains allowing the functional entities to come into close proximity when the complementary domains interact with to each other. The method may be used for generating libraries of templated molecules which may be selected for biological activity.
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Citations
53 Claims
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1-7. -7. (canceled)
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8. A method for synthesising a templated molecule, which is covalently attached to the template which directed the synthesis of the templated molecule and/or covalently attached to the complementary template, said method comprising the steps of:
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a) providing at least one template comprising one or more codons, b) providing a first functional entity attached to a first zipping oligonucleotide capable of reversible interaction with a second zipping oligonucleotide, c) providing one or more building blocks, each building block comprising a further functional entity linked to an anti-codon by a linker, wherein the anti-codon complements a codon of the template, wherein the further functional entity is connected to the second zipping oligonucleotide capable of reversible interaction with the first zipping oligonucleotide attached to the first functional entity provided in step b), and wherein the further functional entity is capable of being chemically connected to the first functional entity provided in step b), d) contacting the components provided in steps a), b), and c) with each other under conditions allowing for i) specific hybridisation of building block anti-codon(s) to the codon(s) of the template(s), ii) dimerization of two zipping oligonucleotides attached to different functional entities, and optionally iii) forming a complementary template comprising functional entity anti-codons, e) allowing a further functional entity of the one or more building blocks provided in step c) to form a chemical connection to the first functional entity provided in step b), and f) obtaining a templated molecule, said templated molecule being covalently attached to the template, which directed the synthesis of the templated molecule and/or covalently attached to the complementary template. - View Dependent Claims (9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53)
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Specification