APPARATUS FOR PRODUCING PROBE CARRIER

• US 20090143251A1
• Filed: 02/03/2009
• Published: 06/04/2009
• Est. Priority Date: 03/28/2001
• Status: Active Grant

• Current Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Original Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Inventors: Okamoto, Tadashi, Okamura, Nobuyuki, Kameyama, Makoto
• Granted Patent: US 8,628,949 B2
• Time in Patent Office: Days
• US Class Current: 506/40
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/0036 Nozzles
• CPC Class Codes: B01J 2219/00378 Piezoelectric or ink jet dispensers
• CPC Class Codes: B01J 2219/00385 Printing
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00698 Measurement and control of process parameters
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/0074 Biological products
• CPC Class Codes: B01L 2200/0684 Venting, avoiding backpressure, avoid gas bubbles
• CPC Class Codes: B01L 2200/142 Preventing evaporation
• CPC Class Codes: B01L 3/0268 using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 60/14 for creating libraries

Abstract

An apparatus for producing a probe carrier in which ambient humidity for a plurality of nozzles of a liquid ejection device for ejecting droplets of solutions containing probes to the surface of a carrier is controlled to 50% or more, preferably 60% or more. A part of solution supply passages to the plurality of nozzles is constructed from gas permeable membranes permitting gas-liquid separation under reduced pressure. These gas permeable membrane portions are gathered and altogether placed under reduced pressure to remove air bubbles from the respective solutions supplied to the plural nozzles and decrease the amount of a dissolved gas, which causes air bubbles to be generated.

Claims

1. An apparatus for producing a probe carrier comprising:

a plurality of chambers, including at least a probe imparting chamber, the probe imparting chamber comprising;

a support device for supporting a carrier;

a liquid ejection head for imparting a plurality of solutions to predetermined positions on the carrier, the plurality of solutions containing probes capable of specifically binding to target substances;

a scan device for scanning the liquid ejection head relative to the support device; and

a chamber for maintaining an environment containing the liquid ejection head at a humidity of 60% or more,

wherein the liquid ejection head comprises a plurality of reservoirs and a plurality of liquid ejection nozzles,

wherein the plurality of reservoirs accommodate a plurality of probe solutions containing probes specifically binding to the target substances,

wherein the plurality of liquid ejection nozzles are those for receiving a supply of the probe solutions from the plurality of reservoirs and imparting droplets of the probe solutions onto the predetermined positions on the carrier,

wherein at least the support device and the liquid ejection head are installed in the environment where the humidity can be maintained at 60% or more before imparting the droplets of the probe solutions and the humidity is maintained at 60% or more while imparting the droplets, and

wherein the chamber has an opening for inserting the carrier into the chamber; and

means for transporting the carrier,

wherein the chambers are arranged around the transport means, and

wherein the transport means transports the carrier for insertion into and removal from the chambers.

2. The apparatus according to claim 1, further comprising a testing chamber for testing a probe impartment state on a surface of the carrier.

3. The apparatus according to claim 1, wherein part of solution supply passages between the plurality of probe solution reservoirs and their respective said nozzles are constructed from hollow tube members, parts of the hollow tube members are made of gas permeable members permitting gas-liquid separation under reduced pressure, and the hollow tube members are passed into a vacuum chamber.

4. The apparatus according to claim 1, wherein the liquid ejection head is equipped with a thermal energy generator that provides thermal energy to a liquid in the nozzle in order to eject the liquid.

5. The apparatus according to claim 1, wherein the liquid ejection head is equipped with a thermal energy generator that provides thermal energy to a liquid in the nozzle to eject the liquid.

6. The apparatus according to claim 1, wherein the probe is a nucleic acid, an oligopeptide, a polypeptide, a protein, an enzyme, a substrate for enzyme, an antibody, an epitope for antibody, an antigen, a hormone, a hormone receptor, a ligand, a ligand receptor, an oligosaccharide, or a polysaccharide.

7. The apparatus according to claim 6, wherein the nucleic acid is DNA, RNA, cDNA (complementary DNA), PNA, an oligonucleotide, or a polynucleotide.

8. The apparatus according to claim 1, wherein the chamber has a gate in the opening, the gate being able to open and close.

9. The apparatus according to claim 1, wherein the droplets are fine droplets having a range of 0.1 pl to 50 pl.