Reagents and methods for detecting analytes
First Claim
Patent Images
1. A reagent for detecting an analyte, the reagent comprising:
- a flavoprotein enzyme;
a mediator selected from the group or a combination thereof, wherein R1, R2, R3, R4, R5, R6, R7, R8, and R9 are the same or different, and are independently selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, aryl, heteroaryl, cyclic, heterocyclic, halo, haloalkyl, carboxy, carboxyalkyl, alkoxycarbonyl, aryloxycarbonyl, aromatic keto, aliphatic keto, alkoxy, aryloxy, nitro, dialkylamino, aminoalkyl, sulfo, dihydroxyboron, and combinations thereof;
at least one surfactant;
a polymer; and
a buffer,wherein at least one of the surfactant and the buffer includes an inorganic salt, the ratio of the total inorganic salt to mediator is less than about 3;
1.
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Abstract
A reagent for detecting an analyte comprises a flavoprotein enzyme, a mediator such as a phenothiazine mediator, at least one surfactant, a polymer and a buffer. The reagent may be used with an electrochemical test sensor that includes a plurality of electrodes.
60 Citations
27 Claims
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1. A reagent for detecting an analyte, the reagent comprising:
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a flavoprotein enzyme; a mediator selected from the group or a combination thereof, wherein R1, R2, R3, R4, R5, R6, R7, R8, and R9 are the same or different, and are independently selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, aryl, heteroaryl, cyclic, heterocyclic, halo, haloalkyl, carboxy, carboxyalkyl, alkoxycarbonyl, aryloxycarbonyl, aromatic keto, aliphatic keto, alkoxy, aryloxy, nitro, dialkylamino, aminoalkyl, sulfo, dihydroxyboron, and combinations thereof; at least one surfactant; a polymer; and a buffer, wherein at least one of the surfactant and the buffer includes an inorganic salt, the ratio of the total inorganic salt to mediator is less than about 3;
1.- View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A reagent for detecting an analyte in a fluid sample, the reagent comprising:
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FAD-glucose dehydrogenase having an activity of from about 0.1 Units/μ
L to about 10 Units/μ
L;a 3-(2′
,5′
-disulfophenylimino)-3H-phenothiazine mediator having a concentration of from about 5 mM to about 120 mM;a surfactant having a concentration of from about 0.05 wt. % to about 0.5 wt. % of the reagent; a hydroxyethyl cellulose polymer having a concentration of from about 0.1 wt. % to about 4 wt. % of the reagent; and a buffer, wherein at least one of the surfactant and the buffer includes an inorganic salt, the ratio of the total inorganic salt to mediator is less than about 2;
1. - View Dependent Claims (9, 10, 11)
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12. An electrochemical test sensor comprising:
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a working electrode having a surface; a counter electrode having a surface; and a reagent coating at least a portion of the surface of the working electrode and at least a portion of the surface of the counter electrode, the reagent comprising a flavoprotein, a phenothiazine or a phenoxazine mediator, a buffer, and at least one surfactant, a polymer and a buffer, wherein at least one of the surfactant and the buffer includes an inorganic salt, the ratio of the total inorganic salt to mediator is less than about 3;
1. - View Dependent Claims (13, 14, 15, 16)
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17. A method of detecting an analyte in a fluid sample, the analyte undergoing a chemical reaction, the method comprising the acts of:
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providing an electrode surface; facilitating flow of the fluid sample to the electrode surface using at least one surfactant; catalyzing the chemical reaction with a flavoprotein enzyme; generating a redox equivalent by the chemical reaction; and transferring the redox equivalent to the electrode surface using a phenothiazine or a phenoxazine mediator, wherein the maximum kinetic performance is less than about 3 seconds. - View Dependent Claims (18, 19, 20, 21)
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22. A method of detecting an analyte in a fluid sample, the method comprising the acts of:
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providing an electrode surface; providing a reagent including a flavoprotein enzyme;
a mediator selected from the groupor a combination thereof wherein R1, R2, R3, R4, R5, R6, R7, R8, and R9 are the same or different, and are independently selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, aryl, heteroaryl, cyclic, heterocyclic, halo, haloalkyl, carboxy, carboxyalkyl, alkoxycarbonyl, aryloxycarbonyl, aromatic keto, aliphatic keto, alkoxy, aryloxy, nitro, dialkylamino, aminoalkyl, sulfo, dihydroxyboron, and combinations thereof;
at least one surfactant; and
a buffer;
the reagent being in contact with the electrode surface;contacting the fluid sample with the reagent; and determining the concentration of the analyte, wherein the maximum kinetic performance is less than about 3 seconds. - View Dependent Claims (23, 24, 25, 26, 27)
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Specification