Methods and apparatus for therapeutic drug monitoring using an acoustic device

• US 20090148856A1
• Filed: 05/02/2006
• Published: 06/11/2009
• Est. Priority Date: 05/02/2005
• Status: Active Grant

• Current Assignee: ProterixBio, Inc.
• Original Assignee: BioScale, Inc.
• Inventors: Srivastava, Alok, Miller, Michael, Masters, Brett P., Lundstrom, Mark, Wang, Wayne U.
• Granted Patent: US 7,611,908 B2
• Time in Patent Office: Days
• US Class Current: 435/7.100
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/1822 using Peltier elements
• CPC Class Codes: B01L 2400/043 magnetic forces
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 2291/0256 Adsorption, desorption, surface mass change, e.g. on biosensors
• CPC Class Codes: G01N 2291/02836 Flow rate, liquid level
• CPC Class Codes: G01N 2291/0427 Flexural waves, plate waves, e.g. Lamb waves, tuning fork, cantilever
• CPC Class Codes: G01N 2800/324 Coronary artery diseases, e.g. angina pectoris, myocardial infarction
• CPC Class Codes: G01N 29/022 Fluid sensors based on microsensors, e.g. quartz crystal-microbalance [QCM], surface acoustic wave [SAW] devices, tuning forks, cantilevers, flexural plate wave [FPW] devices microdevices per se B81B
• CPC Class Codes: G01N 29/036 by measuring frequency or resonance of acoustic waves
• CPC Class Codes: G01N 29/4418 with a model, e.g. best-fit, regression analysis
• CPC Class Codes: G01N 33/54313 the carrier being characterised by its particulate form
• CPC Class Codes: G01N 33/9493 Immunosupressants

Abstract

Methods for therapeutic drug monitoring are provided. A plurality of particles, each of which is coated with a capture agent capable of binding a therapeutic drug of choice is combined with the sample to form a plurality of therapeutic drug-particle complexes. The system also includes a transport arrangement for transporting the sample and/or particles to the sensor surface, and optionally a magnetic field inducing structure constructed and arranged to establish a magnetic field at and adjacent to the sensor surface. The resonant sensor produces a signal corresponding to an amount of therapeutic drug-particle complexes that are bound to the sensor surface.

Claims

1-15. -15. (canceled)

16. A method for detecting one or more immunosuppressants in a sample, the method comprising the steps of:

a) introducing a plurality of particles and a competitor molecule into a fluid chamber, said particles being coated with a first capture agent capable of binding an immunosuppressant, wherein at least one surface of the fluid chamber comprises an acoustic device that has been coated with a second capture agent capable of binding to the competitor molecule, and

b) monitoring signal output by said acoustic device, thereby detecting the immunosuppressant.

17. The method of claim 16, wherein the acoustic device is a flexural plate wave device.

18. The method of claim 16, further comprising adjusting dosage of said immunosuppressant administered to an individual based on the level of said drug in the sample.

19. The method of claim 16, wherein prior to being introduced into the fluid chamber, said plurality of particles has been exposed to the sample and to the competitor molecule.

20. The method of claim 16, wherein the competitor molecule comprises the immunosuppressant bound to a tag and the second capture agent is capable of binding to the tag.

21. The method of claim 20, wherein the tag is biotin.

22. The method of claim 16, wherein the competitor molecule comprises two or more immunosuppressant molecules bound to a carrier, and the second capture agent is capable of binding the immunosuppressant.

23. The method of claim 22, wherein the carrier is a selected from the group consisting of horseradish peroxidase and albumin.

24. The method of claim 16, wherein the particles are magnetic and further comprising creating a magnetic flux in proximity to the acoustic device to attract at least one of the plurality of magnetic particles toward the at least one surface.

25. The method of claim 24, wherein the magnetic flux is removed prior to step b).

26. The method of claim 16, wherein the sample is selected from the group consisting of blood, serum, plasma, cerebrospinal fluid, urine, saliva, and biopsy material.

27. The method of claim 16, wherein the immunosuppressant is selected from the group consisting of cyclosporine, tacrolimus, rapamycin, and mycophenolate mofetil.

28. The method of claim 16, further comprising comparing the signal output of b) to a control.

29. The method of claim 28, wherein the control signal is obtained in the absence of sample.

30. The method of claim 28, wherein the control signal is a standard curve.

31. The method of claim 16, wherein the second capture agent is indirectly bound to said surface.

32. The method of claim 31, wherein said surface is coated with a first member of a binding pair, and the second capture agent is bound to a second member of the binding pair.

33. The method of claim 32, wherein a first member of the binding pair is biotin.

34. The method of claim 16, wherein the first or second capture agent is an antibody.

35. The method of claim 33, wherein a second member of the binding pair is a derivative of avidin.