Disposable mini-bioreactor device and method
First Claim
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1. A cell culture system comprising:
- a container comprising a cylindrical side wall, a closed bottom end and an open top end; and
,a cap comprising a septum and a gas permeable membrane, wherein the cap is adapted to close the top end of the container;
wherein the membrane allows gas exchange between an inside of the container and the exterior of the container, and wherein the septum provides a self-sealing access to the inside of the container after penetration with a conduit.
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Abstract
This invention provides cylindrical cell culture tubes with a cap having both a septum and gas exchange membranes. The culture tubes can be used to inoculate media, culture cells, harvest cells and store cells in the same container with reduced risk of contamination, while facilitating automated handling.
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Citations
19 Claims
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1. A cell culture system comprising:
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a container comprising a cylindrical side wall, a closed bottom end and an open top end; and
,a cap comprising a septum and a gas permeable membrane, wherein the cap is adapted to close the top end of the container; wherein the membrane allows gas exchange between an inside of the container and the exterior of the container, and wherein the septum provides a self-sealing access to the inside of the container after penetration with a conduit. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A cell culture system comprising:
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a media container comprising approximately the dimensions of a standard 50-ml centrifuge tube; and
,a screw-on cap for closure of the container and comprising two or more vent ports, a septum positioned proximal to a first vent port, and a gas permeable membrane positioned proximal to one or more additional vent ports; whereby culture media can be added or withdrawn through the septum, and gasses can be exchanged between an inside of the container and an outside of the container. - View Dependent Claims (11)
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12. A method of culturing cells, the method comprising:
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providing a cylindrical culture container having a screw-on cap, which cap comprises a gas permeable membrane and a septum; placing cell culture media and one or more inocula cells inside the culture tube; allowing the one or more cells to divide in the media to produce progeny cells; passing a conduit through the septum and into the media; removing a sample of the progeny cells, inocula cells or media from the culture tube through a conduit. - View Dependent Claims (13, 14)
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15. A cap for a cylindrical container, comprising
a body structure having an outer surface, a first inner surface configured to interact with a top edge of the cylindrical container, and a second inner surface; -
two or more ports positioned within an area defined by the second inner surface and traversing the body structure; and a self-sealing septum and a gas permeable membrane positioned proximal to the two or more ports. - View Dependent Claims (16, 17, 18, 19)
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Specification