METHODS FOR AFFECTING LIPOSOME COMPOSITION ULTRASOUND IRRADIATION
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Abstract
The present invention provides methods for loading of agents and substances into per-formed liposomes, preferably a suspension of pre-formed liposomes as well as to methods for the controlled quantum (step-wise) release of agents and substances from liposomes. One of the principle features of the methods of the invention is to expose the liposomes to ultrasound irradiation having predefined parameters, resulting in an increase in permeability of the liposomes, thereby permitting, respectively, the loading and/or release of agents and substances into and/or from the liposomes.
28 Citations
88 Claims
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1-68. -68. (canceled)
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69. A method for loading an agent into pre-formed liposomes comprising:
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a. contacting pre-formed liposomes, being of a kind that can increase in permeability by an ultrasound irradiation, with said agent; and b. subjecting the pre-formed liposomes to ultrasound irradiation, said irradiation having parameters being effective to increase permeability of said liposomes; c. said contacting of the pre-formed liposomes with said agent is before or after said ultrasound irradiation. - View Dependent Claims (70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80)
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81. A method for reducing the amount of a substance in a fluid medium or tissue comprising:
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a. contacting the fluid medium or tissue with pre-formed liposomes being of a kind that can increase in permeability by an ultrasound irradiation; b. subjecting the pre-formed liposomes to ultrasound irradiation, said irradiation having parameters effective to increase permeability of said liposomes; c. said contacting of the fluid medium or tissue with the pre-formed liposomes is before or after said ultrasound irradiation. - View Dependent Claims (82)
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83. A method for reducing the level of a substance in a subject, the method comprises:
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a. administering to said subject an amount of pre-formed liposomes, being of a kind that can increase in permeability by an ultrasound irradiation, in a manner permitting contact between said liposomes and said substance; b. subjecting the pre-formed liposomes to ultrasound irradiation, and said ultrasound irradiation having parameters effective to increase permeability of said liposomes. - View Dependent Claims (84, 85, 86, 87)
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88. A method for the controlled quantum release from liposomes comprising an agent stably encapsulated therein and being of a kind that can increase in permeability by an ultrasound irradiation, the method comprises subjecting said liposomes to a series of two or more ultrasound irradiation sessions;
- each ultrasound irradiation having parameters effective to increase permeability of said liposomes thereby.
Specification