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SUBTRACTIVE SINGLE LABEL COMPARATIVE HYBRIDIZATION

  • US 20090155809A1
  • Filed: 02/25/2009
  • Published: 06/18/2009
  • Est. Priority Date: 07/30/2007
  • Status: Active Grant
First Claim
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1. A method of determining a nucleotide sequence difference between nucleic acid in a test sample and nucleic acid in a reference sample, comprising:

  • amplifying nucleic acid from a test sample and amplifying nucleic acid from a reference sample, wherein one of the amplification reactions is conducted using dUTP and not dTTP and the other is conducted using dTTP and not dUTP;

    hybridizing to a nucleic acid array a solution comprising the amplified test sample and amplified reference sample; and

    determining the relative amount of hybridized test and reference nucleic acids bound to the array, wherein a difference in the relative amount of hybridized test and reference nucleic acids bound to the array identifies the presence of a nucleotide sequence difference.

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