Electrokinetic Thermal Cycler and Reactor
First Claim
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1. A reactor comprising:
- (a) a closed loop reactor;
(b) one or more ports to admit reagents into said closed loop, and to withdraw reaction products from said closed loop;
(c) two or more temperature controllers adapted to control the temperature of said closed loop in two or more distinct zones, wherein the temperatures of different zones can differ substantially from one another;
(d) two or more electrodes in electrical contact with the interior of said closed loop, wherein said electrodes are adapted to cause the electrokinetic flow of reagents in solution through said closed loop;
wherein;
(e) said closed loop and said electrodes are adapted to allow the repeated circulation of reagents in solution through said closed loop an arbitrary number of times, as selected by a user, and to allow the solution to repeatedly pass through the different temperature zones an arbitrary number of times, as selected by a user.
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Abstract
Microfluidic devices are disclosed for carrying out cyclic or iterated reactions such as PCR, LDR, and other cyclic or iterated reactions. A microchannel forms a closed loop, through which a reaction mixture may be thermally cycled an arbitrary number of times. Flow is preferably mediated primarily by electrokinetics. Multiple temperature zones may be employed along the course of a single microchannel loop, for example for PCR. Embodiments may be compact, automated, fast, and operable in continuous-flow mode. Real-time reaction monitoring may optionally be used.
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Citations
16 Claims
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1. A reactor comprising:
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(a) a closed loop reactor; (b) one or more ports to admit reagents into said closed loop, and to withdraw reaction products from said closed loop; (c) two or more temperature controllers adapted to control the temperature of said closed loop in two or more distinct zones, wherein the temperatures of different zones can differ substantially from one another; (d) two or more electrodes in electrical contact with the interior of said closed loop, wherein said electrodes are adapted to cause the electrokinetic flow of reagents in solution through said closed loop; wherein; (e) said closed loop and said electrodes are adapted to allow the repeated circulation of reagents in solution through said closed loop an arbitrary number of times, as selected by a user, and to allow the solution to repeatedly pass through the different temperature zones an arbitrary number of times, as selected by a user. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification