PULSED-MULTILINE EXCITATION FOR COLOR-BLIND FLUORESCENCE DETECTION
First Claim
1. A method of identifying sample components comprising:
- (a) obtaining a biological sample;
(b) labeling said sample with one or more fluorophores;
(c) separating components of said sample; and
(d) detecting said sample components with a device wherein said device comprises;
one or more lasers configured to emit two or more excitation lines, each excitation line having a different excitation wavelength;
a timing circuit coupled to the one or more lasers and configured to fire the two or more excitation lines sequentially according to a timing program to produce time-correlated fluorescence emission signals from the sample; and
a non-dispersive detector positioned to collect the time-correlated fluorescence emission signals;
wherein said detector collects time correlated data from said sample comprising fluorescent emissions of the sample as a result of irradiation by the one or more excitation lines.
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Abstract
The present invention provides a technology called Pulse-Multiline Excitation or PME. This technology provides a novel approach to fluorescence detection with application for high-throughput identification of informative SNPs, which could lead to more accurate diagnosis of inherited disease, better prognosis of risk susceptibilities, or identification of sporadic mutations. The PME technology has two main advantages that significantly increase fluorescence sensitivity: (1) optimal excitation of all fluorophores in the genomic assay and (2) “color-blind” detection, which collects considerably more light than standard wavelength resolved detection. Successful implementation of the PME technology will have broad application for routine usage in clinical diagnostics, forensics, and general sequencing methodologies and will have the capability, flexibility, and portability of targeted sequence variation assays for a large majority of the population.
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Citations
11 Claims
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1. A method of identifying sample components comprising:
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(a) obtaining a biological sample; (b) labeling said sample with one or more fluorophores; (c) separating components of said sample; and (d) detecting said sample components with a device wherein said device comprises; one or more lasers configured to emit two or more excitation lines, each excitation line having a different excitation wavelength; a timing circuit coupled to the one or more lasers and configured to fire the two or more excitation lines sequentially according to a timing program to produce time-correlated fluorescence emission signals from the sample; and a non-dispersive detector positioned to collect the time-correlated fluorescence emission signals; wherein said detector collects time correlated data from said sample comprising fluorescent emissions of the sample as a result of irradiation by the one or more excitation lines. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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Specification