Method of selecting a desired protein from a library

• US 20090162841A1
• Filed: 08/03/2005
• Published: 06/25/2009
• Est. Priority Date: 08/06/2004
• Status: Abandoned Application

• Current Assignee: Deutsches Krebsforschungszentrum Stiftung des Offentlichen Rechts
• Original Assignee: Deutsches Krebsforschungszentrum Stifting Des Offentlichen Rechts
• Inventors: Hoheisel, Jorg
• Application Number:
  US11/659,180
• Publication Number:
  US 20090162841A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C07K 16/005 constructed by phage libraries
• CPC Class Codes: C07K 16/30 from tumour cells

Abstract

Described is an improved method of selecting a member of a specific binding pair (sbp) having a desired specify, preferably an antibody, from a library expressing said member of a sbp, preferably a phage-display antibody library.

Claims

1. A method of isolating a member of a specific binding pair (sbp) having a desired binding specificity, comprising the following steps:

(a) incubating a library containing a diverse population of sbp members which are bound to the surface of a microorganism and comprise a binding domain for a complementary member of a sbp (i) with a mixture which contains a complementary sbp (tester) and (ii) with a mixture which does not contain a complementary member of an sbp (driver) or less amounts of the complementary member of the sbp compared to (i) under conditions suitable for binding of the complementary member of an sbp to said binding domain;

(b) washing away unbound microorganisms from step (a) (i) and (a) (ii);

(c) isolating the DNA or RNA from the bound microrganisms of step (b) (i) and (b) (ii);

(d) subjecting the DNA or RNA of step (c) to representational difference analysis (RDA); and

, optionally,

(e) expressing the exponentially amplified DNA or RNA of step (d) to obtain a member of an sbp having the desired specificity.

2. The method of claim 1, wherein said microorganism is a phage.

3. The method of claim 1, wherein said member of the sbp is an antibody and said complementary sbp is an antigen.

4. The method of claim 1, wherein step (d) comprises the following steps:

(d1) attaching specific oligonucleotides (adaptors) to both ends of the tester DNA;

(d2) hybridizing tester DNA and driver DNA together;

([alpha]3) filling in the truncated ends of the hybridized DNAs and, optionally, digesting single-stranded, unhybridized DNA regions; and

(d4) subjecting the DNAs of step (d3) to exponential amplification using the same oligonucleotides as has been used for step (d1) as primers.

5. The method of claim 4, wherein in step (d2) the tester DNA to driver DNA is at any ratio.

6. The method of claim 1, wherein step (d) is repeated at least once.

7. The method of claim 1, wherein exponential amplification is carried out by PCR.

8. A method for identifying differences between at least two complex protein populations comprising providing an sbp obtained by the method of claim 1.

9. The method according to claim 8, wherein said member of an sbp is an antibody.

10. The method according to claim 3 or claim 9, wherein said antibody is a tumor specific antibody.