SAMPLE CONTROL FOR CORRECTION OF SAMPLE MATRIX EFFECTS IN ANALYTICAL DETECTION METHODS
First Claim
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1. A method for determining sample matrix effects of a sample on the detection of a label, the method comprising the steps of:
- (a) contacting a predetermined amount of said label or a different label, with a background sample comprising sample matrix or sample-like matrix,(b) contacting a predetermined amount of said label, or of said different label, with a background-free sample not comprising sample matrix or sample-like matrix or any other compound which is capable of interfering with the detection of the label,(c) detecting said label or said different label in said background sample and said background-free sample, and(d) determining a difference between the detection of said label or said different label in said background sample and in said background-free sample, corresponding to said sample matrix effects.
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Abstract
Methods and systems are described suitable to determine the effects of sample matrix on the detection of a label so as to allow correction for these sample matrix effects when using the label in an analytical detection technique. The method is particularly advantageous for use in a disposable molecular diagnosis cartridge.
22 Citations
22 Claims
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1. A method for determining sample matrix effects of a sample on the detection of a label, the method comprising the steps of:
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(a) contacting a predetermined amount of said label or a different label, with a background sample comprising sample matrix or sample-like matrix, (b) contacting a predetermined amount of said label, or of said different label, with a background-free sample not comprising sample matrix or sample-like matrix or any other compound which is capable of interfering with the detection of the label, (c) detecting said label or said different label in said background sample and said background-free sample, and (d) determining a difference between the detection of said label or said different label in said background sample and in said background-free sample, corresponding to said sample matrix effects. - View Dependent Claims (5, 6, 7, 8, 9, 10, 12, 13)
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2. A method for determining sample matrix effects on the detection of an analyte in a sample, the method comprising the steps of:
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(a) providing a test sample from said sample in which said analyte is to be detected, a background sample comprising sample matrix or sample-like matrix, and a background-free sample, not comprising sample matrix or sample-like matrix, (b) detecting and/or quantifying the analyte in said test sample using a label, (c) detecting said sample matrix effects, by a method comprising the steps of; (i) contacting said background sample with a predetermined amount of said label or a different label, (ii) contacting said background-free sample with a predetermined amount of label or said different label, (iii) detecting said label or said different label in said background sample and in said background-free sample, (iv) determining said sample matrix effects by determining a difference between the detection of said label or said different label in said background sample and said background-free sample, (d) correcting the detection and/or quantification of said analyte in said test sample of step (b) with said sample matrix effects determined in (iv). - View Dependent Claims (3, 4, 11, 14, 15)
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16. A system for determining sample matrix effects of a sample on the detection of a label, comprising:
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(a) means for contacting a predetermined amount of said label or a different label, with a background sample comprising sample matrix or sample-like matrix, (b) means for contacting a predetermined amount of said label, or of said different label, with a background-free sample not comprising sample matrix or sample-like matrix or any other compound which is capable of interfering with the detection of the label, (c) means for detecting said label or said different label in said background sample and said background-free sample, and (d) means for determining a difference between the detection of said label or said different label in said background sample and in said background-free sample, corresponding to said sample matrix effects. - View Dependent Claims (18, 19, 20, 21)
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17. A system for determining sample matrix effects on the detection of an analyte in a sample comprising:
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(a) means for providing a test sample from said sample in which said analyte is to be detected, a background sample comprising sample matrix or sample-like matrix, and a background-free sample, not comprising sample matrix or sample-like matrix, (b) means for detecting and/or quantifying the analyte in said test sample using a label, (c) means for contacting said background sample with a predetermined amount of said label or a different label, (d) means for contacting said background-free sample with a predetermined amount of said label or said different label, (e) means for detecting said label or said different label in said background sample and in said background-free sample, (f) means for determining said sample matrix effects by determining a difference between the detection of said label or said different label in said background sample and said background-free sample, (g) means for correcting the detection and/or quantification of said analyte in said test sample responsive to the means for determining the sample matrix effects.
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22. A disposable cartridge (117) for use in a system for determining sample matrix effects on the detection of an analyte in a sample, comprising:
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a first source (101) of one or more samples selected from the group consisting of the test sample containing said analyte, background sample and background-free sample, and a second source (102) of one or more labels and optionally a third source of additives (110), and means for contacting said background sample with a predetermined amount of said label or a different label, for contacting said background-free sample with a predetermined amount of said label or a different label, and for contacting the test sample with a predetermined amount of said label or a different label, and a window to allow detection of said label or said different label in said test sample, said background sample and said background-free sample.
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Specification