DNA analysis method and DNA analyzer
First Claim
1. A deoxyribonucleic acid (DNA) analysis method comprising the steps of:
- providing a sample containing a DNA to be analyzed and a primer hybridized to the DNA;
carrying out an extension reaction of the primer in the presence of deoxyribonucleotide triphosphates (dNTPs) and a DNA polymerase so that a primer extension reaction product is formed;
converting pyrophosphoric acid formed as a result of the extension reaction into a redox compound; and
electrically detecting the redox compound.
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Accused Products
Abstract
Disclosed is a DNA analysis method and a DNA analyzer whose signal intensity is not lowered even when a material at a higher density is measured. There is supplied dATP, dTTP, dGTP, or dCTP from a dATP solution vessel, a dTTP solution vessel, a dGTP solution vessel, or a dCTP solution vessel, and this causes an extension reaction of a double-stranded DNA immobilized to a bead, to yield pyrophosphoric acid. The pyrophosphoric acid is converted into a redox compound by the actions of a reagent and an enzyme contained in a reaction buffer in a reaction buffer vessel. The redox compound causes a variation in surface potential of a measuring electrode bearing an electrochemically active material immobilized thereto through an insulating molecule, and this variation causes a variation in drain current of a field-effect transistor electrically connected to the measuring electrode. Thus, the extension reaction is detected.
34 Citations
20 Claims
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1. A deoxyribonucleic acid (DNA) analysis method comprising the steps of:
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providing a sample containing a DNA to be analyzed and a primer hybridized to the DNA; carrying out an extension reaction of the primer in the presence of deoxyribonucleotide triphosphates (dNTPs) and a DNA polymerase so that a primer extension reaction product is formed; converting pyrophosphoric acid formed as a result of the extension reaction into a redox compound; and electrically detecting the redox compound. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A DNA analysis system comprising:
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a vessel bearing an immobilized nucleic acid or containing an article bearing an immobilized nucleic acid, wherein the vessel is configured to receive a liquid; one or more measuring electrodes that are in contact with the liquid to be contained in the vessel; an electrochemically active material that is immobilized to a surface of the measuring electrode through an insulating molecule; a reference electrode that is in contact with the liquid; one or more potentiometers that measure interface potentials of the one or more measuring electrodes; a first feeder that feeds dATP or an analogue thereof to the vessel; a second feeder that feeds dGTP or an analogue thereof to the vessel; a third feeder that feeds dCTP or an analogue thereof to the vessel; and a fourth feeder that feeds dTTP or an analogue thereof to the vessel. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20)
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Specification