Sensing of biological molecules using carbon nanotubes as optical labels
First Claim
1. A composition comprising:
- a) a carbon nanoparticle;
b) a solubilizing molecule having (i) a hydrophobic portion attached to the carbon nanoparticle and (ii) a hydrophilic portion for solubilizing the composition and having a linking group; and
c) a biological labeling molecule attached to the linking group.
2 Assignments
0 Petitions
Accused Products
Abstract
Disclosed are methods and materials including carbon nanotubes which have a strong Raman and/or fluorescent signal and which have been modified with an amphiphilic molecule having available functional linking groups for linking to a biological compound. Exemplified are surface-functionalized SWNTs (single walled nanotubes) as highly sensitive bio-labels based on the detection of their spectroscopic Raman signature. By solubilizing the nanotubes with polyethylene glycol (PEG)-containing phospholipids, aqueous-stable as well as biocompatible SWNT labels are produced. Specificity in biological detection is then attained by immobilizing reporting molecules off this PEG layer. Highly selective detection of surface immobilized proteins is achieved with detection limit of ˜10 femtomolar, three orders of magnitude higher than the fluorescent technique. Signal stability upon Raman readout as well as compatibility of the SWNT-tagged proteins to the microarray protocols are also demonstrated, making these biocompatible SWNTs highly attractive as novel, alternative bio-labels for ultrasensitive detection of proteins. When excited with a near infrared laser, the nanoparticles give off a distinctive fluorescence signal.
33 Citations
38 Claims
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1. A composition comprising:
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a) a carbon nanoparticle; b) a solubilizing molecule having (i) a hydrophobic portion attached to the carbon nanoparticle and (ii) a hydrophilic portion for solubilizing the composition and having a linking group; and c) a biological labeling molecule attached to the linking group. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A method for preparing a composition for labeling a biological material, comprising the steps of:
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(a) obtaining a nanoparticle; (b) attaching the nanoparticle to a solubilizing molecule having a hydrophilic portion; and (c) linking a biological labeling molecule to the hydrophilic portion. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23)
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- 24. A method of detecting an analyte using Raman scattering with an optical source exciting a sample and an optical detector detecting changes in light scattered by the sample, comprising the step of labeling a portion of the sample with a specific ligand linked to a carbon nanoparticle.
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32. A method of labeling a cell for detection by fluorescence, comprising the steps of:
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a) contacting the cell with a composition comprising b) a carbon nanoparticle; c) a solubilizing molecule having a hydrophobic portion attached to the carbon nanoparticle and a hydrophilic portion for solubilizing the composition and having a linking group; and d) a biological labeling molecule specific for the cell attached to the linking group; e) forming a complex of the cell and the composition of step (a); f) irradiating the complex with near infrared light; and g) detecting near infrared photoluminescence from the carbon nanoparticle. - View Dependent Claims (33, 34, 35, 36, 38)
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37. A composition comprising:
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a) at least two different carbon nanoparticles; b) solubilizing molecules having (i) a hydrophobic portion attached to the carbon nanoparticles and (ii) a hydrophilic portion for solubilizing the composition and having a linking group; and c) at least two different biological labeling molecule attached to the linking group of respective different nanoparticles.
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Specification