Methods for Preparing Cord Matrix Stem Cells (CMSC) for Long Term Storage and for Preparing a Segment of umbilical cord for cryopreservation
First Claim
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1. A method for preparing cord matrix stem cells (CMSC) for cryopreserving, the method comprising contacting the CMSC with a cryoprotectant and cord blood serum or plasma, wherein the serum or plasma is obtained from a source autologous in origin to the CMSC, and wherein the CMSC are isolated from a plurality of locations along an entire circumference of a transverse section of an umbilical cord.
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Abstract
Methods and kits are provided for preparation of umbilical cord fragments and cells using autologous blood or blood products, and for storage of these materials with autologous cells and blood or blood products in containers having a plurality of separable chambers.
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Citations
37 Claims
- 1. A method for preparing cord matrix stem cells (CMSC) for cryopreserving, the method comprising contacting the CMSC with a cryoprotectant and cord blood serum or plasma, wherein the serum or plasma is obtained from a source autologous in origin to the CMSC, and wherein the CMSC are isolated from a plurality of locations along an entire circumference of a transverse section of an umbilical cord.
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2. (canceled)
- 12. A method of cryopreserving, separately or together, a plurality of types of stem cells from a subject, the method comprising apportioning the stem cells into a separate chamber of a container comprising a plurality of chambers, wherein each of the chambers is separately accessible.
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16. A method of preparing an umbilical cord obtained from an animal subject for cryopreservation, the method comprising:
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preparing a plurality of segments of the cord; dissecting each of the plurality of segments, wherein a plurality of resulting cord fragment preparations are obtained from each of the segments; and cryopreserving separately each of the plurality of fragment preparations, wherein the umbilical cord is cryopreserved.
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- 23. A kit comprising a plurality of chambers wherein each chamber contains at least one cryopreserved material selected from the group of cord matrix stem cells (CMSC) and cord blood cells, wherein the CMSC and cord blood cells are obtained from an autologous source, wherein the chambers comprise separate compartments attached within a container, and each chamber is separably accessible so that within each chamber are provided independently with respect to the remainder of the chambers.
- 24. A kit comprising a plurality of chambers each chamber including cryopreserved cord matrix stem cells (CMSC) and/or cord blood cells, wherein the chambers comprise separate compartments attached within a plastic bag, wherein the CMSC and cord blood cells within each chamber are autologous, wherein each chamber is separately openable and wherein CMSC within each chamber are used independently with respect to the remainder of the chambers.
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27. A method of increasing the number of hematopoietic cells, the method comprising:
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transfecting at least one gene into feeder cells thereby improving ability of the feeder cells to serve as a feeder layer; and culturing the hematopoietic cells with the feeder layer, wherein the number of hematopoietic cells is increased. - View Dependent Claims (28, 29, 30, 31, 32, 33)
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34. A method of preparing feeder cells, the method comprising:
genetically manipulating feeder cells, wherein the genetic manipulating results in improving an ability of the feeder cells to serve as a feeder layer. - View Dependent Claims (35, 36, 37)
Specification