INCREASED SPECIFICITY OF ANALYTE DETECTION BY MEASUREMENT OF BOUND AND UNBOUND LABELS
First Claim
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1. A method for detecting and/or quantifying an analyte in a sample, comprising the steps of:
- a) contacting said sample comprising said analyte with a predetermined amount of label capable of binding to said analyte;
b) detecting the fraction of label bound to said analyte;
whereby the amount of label bound to said analyte is indicative of the presence and/or amount of said analyte in said sample; and
c) detecting the fraction of label not bound to said analyte;
whereby the amount of label not bound to said analyte, deducted from said predetermined amount of label, provides an internal control indicative of the presence and/or amount of said analyte in said sample.wherein said detection step in (b) and (c) is ensured using an optical detection method.
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Abstract
The present invention describes the provision of an internal control in analytical techniques involving labeling of analytes, such as SERRS, for detection of an analyte, particularly a biomolecule in a sample, with improved accuracy.
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Citations
13 Claims
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1. A method for detecting and/or quantifying an analyte in a sample, comprising the steps of:
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a) contacting said sample comprising said analyte with a predetermined amount of label capable of binding to said analyte; b) detecting the fraction of label bound to said analyte;
whereby the amount of label bound to said analyte is indicative of the presence and/or amount of said analyte in said sample; andc) detecting the fraction of label not bound to said analyte;
whereby the amount of label not bound to said analyte, deducted from said predetermined amount of label, provides an internal control indicative of the presence and/or amount of said analyte in said sample.wherein said detection step in (b) and (c) is ensured using an optical detection method. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A system for detecting and/or quantifying the presence of an analyte in a sample, comprising:
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a) means for contacting said sample potentially comprising said analyte with a predetermined amount of label capable of binding to said analyte; b) means for detecting the fraction of label bound to said analyte;
whereby the amount of label bound to said analyte is indicative of the presence and optionally of the amount of analyte in said sample; andc) means for detecting the fraction of label not bound to said analyte;
whereby the amount of label not bound to said analyte, deducted from said predetermined amount of label, provides an internal control indicative of the presence and optionally the amount of analyte in said sample,wherein said detection is ensured using an optical detection method.
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Specification