Polynucleotides for the detection of escherichia coli 0157:h7 and escherichia coli 0157:nm verotoxin producers
First Claim
1. A combination of polynucleotides for amplification and detection of nucleic acid sequences from E. coli O157:
- H7 and/or E. coli O157;
NM which produces verotoxin, said combination selected from the group of;
(d) a combination comprising a first polynucleotide primer comprising at least 7 consecutive nucleotides of the sequence as set forth in SEQ ID NO;
1;
a second polynucleotide primer comprising at least consecutive 7 nucleotides of a sequence complementary to SEQ ID NOs;
1 and a polynucleotide probe comprising at least 7 consecutive nucleotides of the sequence as set forth in SEQ ID NO;
19, or the complement thereof;
(e) a combination comprising a first polynucleotide primer comprising at least 7 consecutive nucleotides of the sequence as set forth in SEQ ID NO;
1;
a second polynucleotide primer comprising at least consecutive 7 nucleotides of a sequence complementary to SEQ ID NO;
1 and a polynucleotide probe comprising at least 7 consecutive nucleotides of the sequence as set forth in SEQ ID NO;
29, or the complement thereof; and
(f) a combination comprising a first polynucleotide primer comprising at least 7 consecutive nucleotides of the sequence as set forth in SEQ ID NO;
1;
a second polynucleotide primer comprising at least consecutive 7 nucleotides of a sequence complementary to SEQ ID NO;
1 and a polynucleotide probe comprising at least 7 consecutive nucleotides of the sequence as set forth in SEQ ID NO;
43, or the complement thereof.
2 Assignments
0 Petitions
Accused Products
Abstract
Polynucleotide primers and probes for the specific detection of E. coli 0157:H7 and/or E. coli 0157:NM which produces verotoxin in samples are provided. The primers and probes can be used in real time diagnostic assays for rapid detection of E. coli 0157:H7 and/or E. coli 0157:NM which produces verotoxin. The primers and probes can be used in real-time diagnostic assays for rapid detection of E. coli 0157:H7 and/or E. coli 0157:NM which produces verotoxin in a variety of situations and are capable of distinguishing E. coli 0157:H7 and/or E. coli 0157:NM which produces verotoxin from other E. coli strains. Kits comprising the primers and probes are also provided.
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Citations
2 Claims
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1. A combination of polynucleotides for amplification and detection of nucleic acid sequences from E. coli O157:
- H7 and/or E. coli O157;
NM which produces verotoxin, said combination selected from the group of;(d) a combination comprising a first polynucleotide primer comprising at least 7 consecutive nucleotides of the sequence as set forth in SEQ ID NO;
1;
a second polynucleotide primer comprising at least consecutive 7 nucleotides of a sequence complementary to SEQ ID NOs;
1 and a polynucleotide probe comprising at least 7 consecutive nucleotides of the sequence as set forth in SEQ ID NO;
19, or the complement thereof;(e) a combination comprising a first polynucleotide primer comprising at least 7 consecutive nucleotides of the sequence as set forth in SEQ ID NO;
1;
a second polynucleotide primer comprising at least consecutive 7 nucleotides of a sequence complementary to SEQ ID NO;
1 and a polynucleotide probe comprising at least 7 consecutive nucleotides of the sequence as set forth in SEQ ID NO;
29, or the complement thereof; and(f) a combination comprising a first polynucleotide primer comprising at least 7 consecutive nucleotides of the sequence as set forth in SEQ ID NO;
1;
a second polynucleotide primer comprising at least consecutive 7 nucleotides of a sequence complementary to SEQ ID NO;
1 and a polynucleotide probe comprising at least 7 consecutive nucleotides of the sequence as set forth in SEQ ID NO;
43, or the complement thereof.
- H7 and/or E. coli O157;
-
2-79. -79. (canceled)
Specification