Polynucleotides for the detection of escherichia coli 0157:h7 and escherichia coli 0157:nm verotoxin producers

• US 20090170076A1
• Filed: 05/20/2005
• Published: 07/02/2009
• Est. Priority Date: 05/20/2004
• Status: Active Grant

• Current Assignee: AES Chemunex SA (Compagnie Merieux Alliance SAS)
• Original Assignee: AES Chemunex SA (Compagnie Merieux Alliance SAS)
• Inventors: Taylor, Gregory, Cote, Yvan, Hebert, Alexandre
• Granted Patent: US 8,088,572 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C07K 14/245 Escherichia (G)
• CPC Class Codes: C12Q 1/689 for bacteria

Abstract

Polynucleotide primers and probes for the specific detection of E. coli 0157:H7 and/or E. coli 0157:NM which produces verotoxin in samples are provided. The primers and probes can be used in real time diagnostic assays for rapid detection of E. coli 0157:H7 and/or E. coli 0157:NM which produces verotoxin. The primers and probes can be used in real-time diagnostic assays for rapid detection of E. coli 0157:H7 and/or E. coli 0157:NM which produces verotoxin in a variety of situations and are capable of distinguishing E. coli 0157:H7 and/or E. coli 0157:NM which produces verotoxin from other E. coli strains. Kits comprising the primers and probes are also provided.

Claims

1. A combination of polynucleotides for amplification and detection of nucleic acid sequences from E. coli O157:

H7 and/or E. coli O157;

NM which produces verotoxin, said combination selected from the group of;

(d) a combination comprising a first polynucleotide primer comprising at least 7 consecutive nucleotides of the sequence as set forth in SEQ ID NO;

1;

a second polynucleotide primer comprising at least consecutive 7 nucleotides of a sequence complementary to SEQ ID NOs;

1 and a polynucleotide probe comprising at least 7 consecutive nucleotides of the sequence as set forth in SEQ ID NO;

19, or the complement thereof;

(e) a combination comprising a first polynucleotide primer comprising at least 7 consecutive nucleotides of the sequence as set forth in SEQ ID NO;

1;

a second polynucleotide primer comprising at least consecutive 7 nucleotides of a sequence complementary to SEQ ID NO;

1 and a polynucleotide probe comprising at least 7 consecutive nucleotides of the sequence as set forth in SEQ ID NO;

29, or the complement thereof; and

(f) a combination comprising a first polynucleotide primer comprising at least 7 consecutive nucleotides of the sequence as set forth in SEQ ID NO;

1;

a second polynucleotide primer comprising at least consecutive 7 nucleotides of a sequence complementary to SEQ ID NO;

1 and a polynucleotide probe comprising at least 7 consecutive nucleotides of the sequence as set forth in SEQ ID NO;

43, or the complement thereof.

2-79. -79. (canceled)