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METHOD FOR MAKING AVAILABLE A PRIMING OLIGONUCLEOTIDE

  • US 20090170168A1
  • Filed: 02/20/2009
  • Published: 07/02/2009
  • Est. Priority Date: 08/27/2004
  • Status: Active Grant
First Claim
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1. A method for making available a priming oligonucleotide for extension in the presence of a target nucleic acid, the method comprising the steps of:

  • (a) providing the priming oligonucleotide to a reaction mixture containing the target nucleic acid, the priming oligonucleotide having a cap hybridized to a region at the 3′

    -end thereof, wherein the 5′

    -terminal base of the cap is complementary to the 3′

    -terminal base of the priming oligonucleotide, and wherein the cap is modified to prevent the initiation of DNA synthesis therefrom;

    (b) hybridizing the priming oligonucleotide to a target sequence contained within the target nucleic acid, thereby displacing the cap from the priming oligonucleotide; and

    (c) extending the priming oligonucleotide in a primer extension reaction with a DNA polymerase to give a DNA primer extension product complementary to the target sequence.

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