Cell stimulating device and cell stimulating method

• US 20090170178A1
• Filed: 04/22/2004
• Published: 07/02/2009
• Est. Priority Date: 04/22/2003
• Status: Abandoned Application

• Current Assignee: Riken
• Original Assignee: Riken
• Inventors: Itoh, Kouichi, Ozaki, Miwako
• Application Number:
  US10/553,601
• Publication Number:
  US 20090170178A1
• Time in Patent Office: Days
• US Class Current: 435/173.800
• CPC Class Codes: C12M 23/10 Petri dish crystallising dishes B01L3/06
• CPC Class Codes: C12M 35/02 Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves

Abstract

It is an object of the present invention to provide an electrical stimulating device for efficiently providing direct electrical stimulation to a large number of nerve cells in vitro without causing any injuries to such cells. The present invention provides a cell stimulating device which comprises: a first electrode serving as a positive or negative electrode that extends from one side of a culture vessel that is used for accommodating cultured cells to a point at which the first electrode is not in contact with the cultured cells or is in contact with the surfaces of the cultured cells; and a second electrode serving as a negative or positive electrode that extends from the other side of the culture vessel to a point at which the second electrode is not in contact with the cultured cells or is in contact with the surfaces of the cultured cells, wherein an electric field for stimulating cells is formed via the first electrode and the second electrode.

Claims

1. A cell stimulating device which comprises:

a first electrode serving as a positive or negative electrode that extends from one side of a culture vessel that is used for accommodating cultured cells to a point at which the first electrode is not in contact with the cultured cells or is in contact with the surfaces of the cultured cells; and

a second electrode serving as a negative or positive electrode that extends from the other side of the culture vessel to a point at which the second electrode is not in contact with the cultured cells or is in contact with the surfaces of the cultured cells, wherein an electric field for stimulating cells is formed via the first electrode and the second electrode.

2. The cell stimulating device according to claim 1 which comprises:

a first electrode serving as a positive or negative electrode that extends from either a top or a side portion of a culture vessel that is used for accommodating cultured cells to a point at which the first electrode is not in contact with the cultured cells or is in contact with the surfaces of the cultured cells; and

a second electrode serving as a negative or positive electrode that extends from the other top or side portion of the culture vessel to a point at which the second electrode is not in contact with the cultured cells or is in contact with the surfaces of the cultured cells, wherein an electric field for stimulating cells is formed via the first electrode and the second electrode.

3. The cell stimulating device according to claim 1 wherein said first electrode is a circular ring electrode.

4. The cell stimulating device according to claim 1 wherein said second electrode is a single-point electrode.

5. The cell stimulating device according to claim 1 wherein said second electrode is a multi-point electrode that is a plurality of electrodes.

6. The cell stimulating device according to claim 1 wherein said second electrode is an electrode formed with mesh-sheet.

7. The cell stimulating device according to claim 1 wherein said second electrode is a sheet electrode comprising a multi-point electrode.

8. A method for electrically stimulating cells with the use of the cell stimulating device of claim 1, wherein an electric field is formed by said first and second electrodes and cultured cells are stimulated in the electric field.

9. The method for electrically stimulating cells according to claim 8 wherein the cultured cells are nerve cells.