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METHOD OF SEQUENCING NUCLEIC ACIDS USING ELABORATED NUCLEOTIDE PHOSPHOROTIOLATE COMPOUNDS

  • US 20090171078A1
  • Filed: 11/20/2008
  • Published: 07/02/2009
  • Est. Priority Date: 11/20/2007
  • Status: Abandoned Application
First Claim
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1. A method of synthesizing a nucleic acid comprising;

  • hybridizing a primer to a template;

    polymerase extending the primer with an elaborated mono-nucleotide phosphorothiolate compound to form an extension product, wherein the elaborated mono-nucleotide phosphorothiolate compound comprises a 3′

    C—

    O—

    PO2

    S—

    X group;

    cleaving the 3′

    C—

    O—

    PO2

    S—

    X group with a phosphorothiolate cleaving agent, wherein the S—

    X are removed from the extension product, to leave a 3′

    PO4 group;

    hydrolyzing the 3′

    PO4 group with a phosphate removing agent to leave a 3′

    OH; and

    ,repeating (b)-(d) to synthesize the nucleic acid.

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