Administration of cells and cellular extracts for rejuvenation

• US 20090175927A1
• Filed: 05/11/2007
• Published: 07/09/2009
• Est. Priority Date: 05/11/2006
• Status: Active Grant

• Current Assignee: Regenics AS
• Original Assignee: Regenics AS
• Inventors: Gammelsaeter, Runhild, Remmereit, Jan
• Granted Patent: US 8,075,920 B2
• Time in Patent Office: Days
• US Class Current: 424/449
• CPC Class Codes: A61K 31/7088 Compounds having three or more nucleosides or nucleotides
• CPC Class Codes: A61K 35/36 Skin; Hair; Nails; Sebaceous glands; Cerumen; Epidermis; Epithelial cells; Keratinocytes; Langerhans cells; Ectodermal cells islets of Langerhans A61K35/39
• CPC Class Codes: A61K 35/60 Fish, e.g. seahorses; Fish eggs
• CPC Class Codes: A61K 38/02 Peptides of undefined number of amino acids; Derivatives thereof
• CPC Class Codes: A61K 45/06 Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• CPC Class Codes: A61K 8/606 Nucleosides; Nucleotides; Nucleic acids
• CPC Class Codes: A61K 8/64 Proteins; Peptides; Derivatives or degradation products thereof
• CPC Class Codes: A61K 8/65 Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
• CPC Class Codes: A61K 8/9789 Magnoliopsida [dicotyledons]
• CPC Class Codes: A61K 8/981 of mammals or bird
• CPC Class Codes: A61K 8/982 Reproductive organs; Embryos, Eggs
• CPC Class Codes: A61K 8/987 of species other than mammals or birds
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 17/16 Emollients or protectives, e.g. against radiation
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 21/00 Drugs for disorders of the muscular or neuromuscular system
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 31/00 Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• CPC Class Codes: A61P 39/06 Free radical scavengers or antioxidants
• CPC Class Codes: A61P 43/00 : Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61Q 19/00 : Preparations for care of the skin
• CPC Class Codes: A61Q 19/004 : Aftersun preparations
• CPC Class Codes: A61Q 19/06 : for countering cellulitis
• CPC Class Codes: A61Q 19/08 : Anti-ageing preparations

Abstract

The invention describes methods and agents for improving cosmetic appearance, for promoting, improving or restoring health of cells and tissues, preferably skin, and more preferably, for restoring aged or damaged skin to a healthy appearance. This invention relates to the use of cells and cellular extracts in rejuvenation and healing technologies thereby improving healing and regeneration of all bodily tissues and organs. The present invention relates to compositions and methods of managing, preventing, and treating scars. The invention also relates to prevention of deterioration, damage and malfunction of cells and tissues, and to promote, improve or exceed cellular function in order to promote, improve and exceed appearance, vitality and health. In some embodiments, the invention relates to compositions of cells, eggs, cell extracts, egg extracts, and extract components such as purified nucleic acids, polypeptides, lipids, carbohydrates or other natural products. Said components can also be synthesized. In other embodiments, the cells are differentiable cells preferably stem cells or eggs. In some embodiments, compositions are used in a method that comprises application of compositions to tissues, including skin and/or wounds after removal of tissue surface layers. In other embodiments, the invention relates to methods of dedifferentiation of cells and/or dedifferentiation followed by differentiation. In other embodiments, the invention relates to managing, preventing, and treating skin diseases.

Claims

1. A composition comprising i) a cellular component selected from the group consisting of differentiable cells, differentiable cell cellular extracts and an egg cellular extract or combinations thereof and ii) a lipid component from a different source than said cells or eggs.

2. The composition of claim 1 wherein said composition further comprises purified or synthetic nucleic acid sequences, polypeptides, carbohydrates, lipids, signaling substance or natural products or combinations thereof.

3. The composition of claim 1 wherein said differentiable cells are selected from the group consisting of embryonic stem cells, embryonic germ cells, and adult stem cells.

4. The composition of claim 1, further comprising an epigenetic inhibitor.

5. The composition of claim 1, wherein said composition is provided in a cream, gel, emulsion, ointment, spray, powder or lotion.

6. A composition comprising a cellular component selected from the group consisting of differentiable cells, differentiable cell cellular extracts and an egg cellular extract and combinations thereof in a cream, gel, spray, emulsion, solid, plastic or matrix, ointment, powder or lotion suitable for topical administration.

7. The composition of claim 6 wherein said composition further comprises purified or synthetic nucleic acid sequences, polypeptides, or natural products or combinations thereof.

8. The composition of claim 6 wherein said composition further comprises an epigenetic inhibitor.

9. The composition of claim 6, wherein said composition further comprises a lipid or protein fraction from a source other than said differentiable cells.

10. The composition of claim 9 wherein the lipid fraction comprises squalene, an aliphatic wax, a sterol ester, a diol ester, a triglyceride, or a sterols or combinations thereof.

11. The composition of claim 9 wherein said lipid or protein fraction is derived from egg from fish, shrimp, sea urchin, frog or fish roe or both.

12. The composition of claim 6 wherein said composition further comprises keratin or filaggrin.

13. The composition of claim 6 wherein said composition further comprises glutamine, antiinfective agents, anti-inflammatory agents, antioxidants and/or nicotinamide.

14. The composition of claim 13 wherein aid antioxidant is vitamin A, C, D, or E or combinations thereof.

15. The composition of claim 6, wherein said gel comprises a compound selected from the group consisting of hyaluronic acid and chitosan.

16. The composition of claim 6 wherein said spray is an aerosol.

17. The composition of claim 6 wherein said spray dries on the skin.

18. The composition of claim 6 wherein said spray composition comprises gel-forming components.

19. A method for treating the skin of a subject comprising:

applying a composition comprising a cellular component selected from the group consisting of differentiable cells, differentiable cell cellular extracts and an egg cellular extract and combinations thereof to the skin of said subject.

20. The method of claim 19, further comprising the step of removing skin tissue by chemicals, a laser or physical force prior to said applying step.

21. The method of claim 19, wherein said composition is applied under conditions so that the appearance of the skin is improved, wherein improving the appearance of skin is selected from the group consisting of improving the appearance of a scar, improving the appearance of skin with wrinkles or sun damage, and improving the appearance of skin damaged by skin disorders.

22. The method of claim 19, wherein said subject has uneven or damaged skin and said composition is applied to said uneven or damaged skin under conditions that such skin is rejuvenated

23. A method for the topical administration of a composition comprising a cellular component selected from the group consisting of differentiable cells, differentiable cell cellular extracts and an egg cellular extract and combinations thereof comprising:

a) providing a composition comprising a ca cellular component selected from the group consisting of differentiable cells, differentiable cell cellular extracts and an egg cellular extract and combinations thereof and a subject having skin; and

b) applying said composition to said skin.

24. The method of claim 23 wherein said cellular extract selected from the group consisting of differentiable cell cellular extracts and egg cellular extracts or combinations thereof is effective as a nutrient to a cell of said skin.

25. The method of claim 23, wherein said composition is applied to a wound in said skin.

27. The method of claim 25 wherein said wound dressing comprises:

i) a waterproof layer;

ii) a nutrient gel layer comprising a cellular component selected from the group consisting of differentiable cells, differentiable cell cellular extracts and an egg cellular extract and combinations thereof.

26. A method for the topical administration of a composition comprising a cellular component selected from the group consisting of differentiable cells, differentiable cell cellular extracts and an egg cellular extract and combinations thereof comprising:

i) providing

a) a composition comprising a cellular component selected from the group consisting of differentiable cells, differentiable cell cellular extracts and an egg cellular extract and combinations thereof,

b) a subject having a wound in skin and

c) wound dressing

ii) applying said differentiable cells, cell extracts, egg extracts, components of cell extracts, egg extracts to said wound; and

iii) covering said wound with said wound dressing.

28. A composition for stimulating the growth of cells comprising an effective amount of a cellular fraction of an embryonic stem cell, progenitor cell, germ cell, fish, shrimp, sea urchin egg and/or frog egg.