Microfluidic device and methods of using same
First Claim
1. A microfluidic device comprising:
- a fluidic channel having a first end and a second end in fluid communication with each other through said channel;
an inlet in communication with said channel, said inlet for introducing a sample fluid; and
,a valve, said valve, when actuated, causing said first channel end to become fluidically separated from said second channel end;
wherein when said sample fluid is introduced into said fluidic channel, and said valve is actuated, said sample fluid is divided into separate sample fluid portions.
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Accused Products
Abstract
A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.
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Citations
13 Claims
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1. A microfluidic device comprising:
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a fluidic channel having a first end and a second end in fluid communication with each other through said channel; an inlet in communication with said channel, said inlet for introducing a sample fluid; and
,a valve, said valve, when actuated, causing said first channel end to become fluidically separated from said second channel end; wherein when said sample fluid is introduced into said fluidic channel, and said valve is actuated, said sample fluid is divided into separate sample fluid portions. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method of analyzing a sample fluid for a reaction to a condition comprising the steps of:
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(i) providing a microfluidic device, said microfluidic device comprising; a fluidic channel having a first end and a second end in fluid communication with each other through said channel; an inlet in communication with said channel, said inlet for introducing said sample fluid; and
,a valve, said valve, when actuated, causing said first channel end to become fluidically separated from said second channel end; wherein when said sample fluid is introduced into said fluidic channel, and said valve is actuated, said sample fluid is divided into separate sample fluid portions; (ii) introducing said sample fluid into said inlet, said sample fluid filling said first and second channel ends; (iii) actuating said valve to separate said sample fluid into said separate portions within said first and second channel ends; (iv) exposing said sample fluid to said conditions; (v) analyzing said sample fluid to determine whether said conditions produced said reaction. - View Dependent Claims (8, 9, 10, 11, 12, 13)
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Specification