COENZYME-BINDING GLUCOSE DEHYDROGENASE

• US 20090176262A1
• Filed: 03/03/2009
• Published: 07/09/2009
• Est. Priority Date: 12/24/2002
• Status: Abandoned Application

• Current Assignee: Hirokazu Sanada, Hironori Omura, Kenji Kano, Mika Kuyama, Seiya Tsujimura, Takako Yada, Tetsunari Morita, Tokuji Ikeda
• Original Assignee: Hirokazu Sanada, Hironori Omura, Kenji Kano, Mika Kuyama, Seiya Tsujimura, Takako Yada, Tetsunari Morita, Tokuji Ikeda
• Inventors: Omura, Hironori, Yada, Takako, Kano, Kenji, Morita, Tetsunari, Sanada, Hirokazu, Ikeda, Tokuji, Kuyama, Mika, Tsujimura, Seiya
• Application Number:
  US12/396,724
• Publication Number:
  US 20090176262A1
• Time in Patent Office: Days
• US Class Current: 435/14
• CPC Class Codes: C12N 9/0004 Oxidoreductases (1.)
• CPC Class Codes: C12Q 1/32 involving dehydrogenase
• CPC Class Codes: C12Q 1/54 involving glucose or galactose

Abstract

The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level. Therefore, it became possible to provide an enzyme having a high utility, such as an ability of being used for modifying a material in the fields of pharmaceuticals, clinical studies and food products.

Claims

1-19. -19. (canceled)

20. A biosensor for measuring glucose comprising soluble flavin compound-binding glucose dehydrogenase obtainable from Aspergillus terreus, or a mutant thereof which results from a deletion, substitution or addition of one amino acid residue, which:

(a) catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor;

(b) has a 5% or less specific activity to maltose relative to the enzymatic activity to glucose; and

(c) is inhibited by 1,10-phenanthroline.

21. The biosensor of claim 20, wherein ferricyanide is employed at a final concentration of 2 mM to 500 mM.