Porous Particle Reagent Compositions, Devices, and Methods for Biosensors
First Claim
1. A reagent composition, for determining the concentration of an analyte in a sample, comprising:
- about 1% to about 30% (w/w) of an about 20% to an about 50% (w/w) suspension of porous particles, the particles having an average diameter from 0.05 micrometer to 10 micrometer and a void volume of at least 20% (v/v);
about 0.1% to about 10% (w/w) of at least one polymeric material; and
about 0.1 active units to about 10 active units of at least one enzyme system per microliter of the reagent composition.
2 Assignments
0 Petitions
Accused Products
Abstract
A reagent composition for a biosensor sensor strip is disclosed that provides for rapid rehydration after drying. The composition includes porous particles and is preferably formed as a colloidal suspension. The dried reagent composition including porous particles may provide analytically useful output from the sensor strip in a shorter time period than observed from dried reagent compositions using solid particles. The output signal from the porous particle compositions may be correlated to the analyte concentration of a sample within about two seconds. In this manner, an accurate concentration determination of an analyte concentration in a sample may be obtained in less time than from sensor strips including conventional compositions. The reagent composition including the porous particles also may allow for the redox reaction between the reagents and the analyte to reach a maximum kinetic performance in a shorter time period than observed from conventional sensor strips.
22 Citations
41 Claims
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1. A reagent composition, for determining the concentration of an analyte in a sample, comprising:
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about 1% to about 30% (w/w) of an about 20% to an about 50% (w/w) suspension of porous particles, the particles having an average diameter from 0.05 micrometer to 10 micrometer and a void volume of at least 20% (v/v); about 0.1% to about 10% (w/w) of at least one polymeric material; and about 0.1 active units to about 10 active units of at least one enzyme system per microliter of the reagent composition. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. An electrode for a biosensor, comprising:
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at least one conductor; and at least one reagent composition disposed on the conductor, the reagent composition comprising; porous particles having an average diameter from 0.05 micrometer to 10 micrometer and a void volume of at least 20% (v/v); from about 0.5% to about 10% (w/w) of at least one mediator; at least one polymeric material; and at least one enzyme system. - View Dependent Claims (11, 12, 13, 14)
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15. A biosensor sensor strip, for determining the concentration of an analyte in a sample, comprising:
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a sensor base at least partially covered by a lid; at least one reservoir formed by the sensor base, where the at least one reservoir encloses at least two conductors disposed on the base; at least one reagent composition on at least one of the conductors to form a working electrode, the at least one reagent composition comprising, about 0.1 active unit to about 10 active units of at least one enzyme system per microliter of the at least one reagent composition; about 0.5% to about 10% (w/w) of at least one mediator; and at least one polymeric material, where the reservoir and the at least one reagent composition provide a maximum kinetic performance of a redox reaction of an analyte with the at least one reagent composition within less than about three seconds of introducing a sample to the sensor strip, and where the maximum kinetic performance is determined with a gated amperometric pulse sequence having at least five duty cycles, and where each excitation of the duty cycles is 0.4 second in duration, each relaxation of the duty cycles is one second in duration, the relaxations are provided by an open circuit, at least three output current values are measured during each excitation, the excitations have a substantially constant potential of 250 mV, and the sample temperature is 23°
C.
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16-24. -24. (canceled)
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25. A biosensor system, for determining the concentration of an analyte in a sample, comprising:
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a support means for supporting at least two conductors; a reaction means for selectively performing a redox reaction on an analyte, where the reaction means include at least one polymeric material; a measurement means for measuring a rate of redox of the analyte, where the measurement means includes at least two conductors; and where the measurement means measures a rate of the redox reaction at a maximum kinetic performance within less than about three seconds of introducing a sample to the reaction means, and where the maximum kinetic performance is determined with a gated amperometric pulse sequence having at least five duty cycles, and where each excitation of the duty cycles is 0.4 seconds in duration, each relaxation of the duty cycles is one second in duration, the relaxations are provided by an open circuit, at least three output current values are measured during each excitation, the excitations have a substantially constant potential of 250 mV, and the sample temperature is 23°
C.
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26-36. -36. (canceled)
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37. A method of determining the concentration of an analyte in a sample, comprising:
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introducing an aqueous sample including at least one analyte to a reagent composition, the reagent composition comprising, porous particles having an average diameter from 0.05 micrometer to 10 micrometer and a void volume of at least 20% (v/v) and at least one polymeric material; rehydrating the reagent composition with the aqueous sample; applying an input signal to the aqueous sample; measuring at least one output signal current value within about 0.4 to about 5 seconds of introducing the aqueous sample to the reagent composition; and determining the concentration of the at least one analyte in the aqueous sample from the at least one output signal current value. - View Dependent Claims (38, 39, 40)
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41-48. -48. (canceled)
Specification