Mesoderm And Definitive Endoderm Cell Populations

• US 20090181453A1
• Filed: 11/17/2008
• Published: 07/16/2009
• Est. Priority Date: 05/17/2002
• Status: Abandoned Application

• Current Assignee: Mount Sinai School of Medicine of New York University
• Original Assignee: Icahn School of Medicine at Mount Sinai
• Inventors: Keller, Gordon, Gadue, Paul
• Application Number:
  US12/272,308
• Publication Number:
  US 20090181453A1
• Time in Patent Office: Days
• US Class Current: 435/325
• CPC Class Codes: A61P 1/16 for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 21/00 Drugs for disorders of the muscular or neuromuscular system
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: A61P 7/00 Drugs for disorders of the blood or the extracellular fluid
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system
• CPC Class Codes: C12N 2500/90 Serum-free medium, which may still contain naturally-sourced components
• CPC Class Codes: C12N 2501/11 Epidermal growth factor [EGF]
• CPC Class Codes: C12N 2501/115 Basic fibroblast growth factor (bFGF, FGF-2)
• CPC Class Codes: C12N 2501/12 Hepatocyte growth factor [HGF]
• CPC Class Codes: C12N 2501/148 Transforming growth factor alpha [TGF-a]
• CPC Class Codes: C12N 2501/155 Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
• CPC Class Codes: C12N 2501/16 Activin; Inhibin; Mullerian inhibiting substance
• CPC Class Codes: C12N 2501/385 of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
• CPC Class Codes: C12N 2501/40 Regulators of development
• CPC Class Codes: C12N 2501/415 Wnt; Frizzeled
• CPC Class Codes: C12N 2506/02 from embryonic cells
• CPC Class Codes: C12N 2506/03 from non-embryonic pluripotent stem cells
• CPC Class Codes: C12N 5/0603 Embryonic cells production of embryos, nuclear transfer A01K67/027; Embryoid bodies
• CPC Class Codes: C12N 5/0606 Pluripotent embryonic cells, e.g. embryonic stem cells [ES] embryonic germ cells C12N5/0611, induced pluripotent stem cells C12N5/0696
• CPC Class Codes: C12N 5/0607 : Non-embryonic pluripotent stem cells, e.g. MASC induced pluripotent stem cells C12N5/0696
• CPC Class Codes: C12N 5/067 : Hepatocytes

Abstract

The present invention provides cell populations that are enriched for mesendoderm and mesoderm, and cell populations that are enriched for endoderm. The cell populations of the invention are useful for generating cells for cell replacement therapy.

Claims

1. A developmentally normal embryonic stem cell in which a nucleic acid encoding a first selectable marker is operably linked to brachyury regulatory elements and wherein one brachyury allele is inactivated by said nucleic acid and said first selectable marker is expressed.

2. The embryonic stem cell of claim 1 in which a second nucleic acid encoding a selectable marker is present in the HNF3β

locus.

3. The stem cell of claim 2 in which the second selectable marker is human CD4.

4. The stem cell of claim 1 in which the first selectable marker is green fluorescent protein.

5. A developmentally normal embryonic stem cell in which a nucleic acid encoding green fluorescent protein is operably linked to brachyury regulatory elements, and wherein one brachyury allele is inactivated by said nucleic acid encoding green fluorescent protein.