Methods For Rapid Forensic Analysis Of Mitochondrial DNA And Characterization Of Mitochondrial DNA Heteroplasmy
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention provides methods for rapid forensic analysis of mitochondrial DNA and methods for characterizing heteroplasmy of mitochondrial DNA, which can be used to assess the progression of mitochondrial diseases.
-
Citations
126 Claims
-
1-28. -28. (canceled)
-
29. A method of identifying a nucleic acid sequence from a human sample, comprising:
-
a) contacting nucleic acid from a human sample with two or more oligonucleotide primers that hybridize to sequence regions of said nucleic acid that are conserved among different bioagents, wherein said conserved sequence regions flank a variable sequence region to produce an amplification product; b) determining the base composition of said amplification product; and c) identifying said nucleic acid sequence from a human sample by comparing said determined base composition to a database of base compositions from a plurality of different bioagents. - View Dependent Claims (30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101)
-
-
102. A system, comprising:
-
a) a nucleic acid amplification component; b) a base composition determination component; and c) a base composition identification component comprising a database of base compositions from a plurality of bioagents wherein said bioagents comprise human bioagents. - View Dependent Claims (103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126)
-
Specification