IMAGE SCANNING APPARATUS AND METHOD

• US 20090185167A1
• Filed: 01/18/2008
• Published: 07/23/2009
• Est. Priority Date: 01/18/2008
• Status: Active Grant

• Current Assignee: FFEI Limited (XAAR, PLC)
• Original Assignee: FFEI Limited (XAAR, PLC)
• Inventors: Gouch, Martin Philip
• Granted Patent: US 7,645,971 B2
• Time in Patent Office: Days
• US Class Current: 356/73
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G02B 21/245 using auxiliary sources, detectors

Abstract

An image scanning apparatus comprises a time delay integration sensor for obtaining first image information from a target and a scan device for causing relative motion between the time delay integration sensor and the target. The image scanning apparatus is characterized by detector array for obtaining second image information from a target, wherein the first image information corresponds to a first portion of light received from the target and the second image information corresponds to a second portion of light received from the target.

Claims

1. An image scanning apparatus comprising:

a time delay integration sensor for obtaining first image information from a target; and

a scan device for causing relative motion between the time delay integration sensor and the target to enable an image of the target to be generated from a scan of the target; and

a processor;

the image scanning apparatus characterized by;

a detector array for obtaining second image information from a target;

wherein the first image information corresponds to a first portion of light received from the target and the second image information corresponds to a second portion of light received from the target, the first and second portions being separated in at least one of the following ways;

spatially, chromatically or temporally; and

wherein the processor is adapted to use said second information to calculate a focus position for obtaining the first image information.

2. The image scanning apparatus of claim 1, wherein the first and second portions respectively correspond to at least one of:

different spatial areas of the target;

different wavelengths of light received from the target; and

light received from the target at different time periods.

3. The image scanning apparatus of claim 1, further comprising:

a focusing device adapted to modify the focus between the time delay integration sensor and the target;

wherein the processor is adapted to control the focusing device and;

the second image information is used by the processor to select a focus position for the focusing device.

4. The image scanning apparatus of claim 1, further comprising a fluorescence exciter source configured to irradiate the target and cause the target to fluoresce.

5. The image scanning apparatus of claim 4, wherein the exciter source comprises an ultra violet source.

6. The image scanning apparatus of claim 4, wherein the exciter source is located below the sample to provide trans-illumination.

7. The image scanning apparatus of claim 6, further comprising a filter positioned on the optical path between the target and the time delay integration sensor adapted to prevent predetermined spectral bands of radiation from impinging on the time delay integration sensor.

8. The image scanning apparatus of claim 1, wherein the optical path length from the target to the detector array equals the optical path length from the target to the time delay integration sensor.

9. The image scanning apparatus of claim 4, wherein the detector array comprises a red, green, blue (RGB) detector array.

10. The image scanning apparatus of claim 1, further comprising at least one light redirection device configured to redirect light from the target to at least one of the time delay integration sensor and the detector array.

11. The image scanning apparatus of claim 10, wherein the at least one light redirection device comprises a dichroic beam splitter.

12. The image scanning apparatus of claim 10, wherein the at least one light redirection device comprises a mirror positioned along the optical path between the target and the detector array such that a central portion of the light received from the target is directed toward the time delay integration sensor and a peripheral portion of the light received from the target is directed toward the detector array.

13. The image scanning apparatus of claim 10, wherein the light redirection device comprises an off-axis mirror, located a predetermined distance from an optical path associated with one of the time delay integration sensor or the detector array so as to redirect light to the other of the detector array or time delay integration sensor.

14. The image scanning apparatus of claim 1, further comprising at least one light source adapted to illuminate the target using at least one of epi-illumination or trans-illumination.

15. The image scanning apparatus of claim 1, wherein the detector array is a linear detector array configured to capture a scan line of the target.

21. The image scanning apparatus of claim 1, wherein the second image information comprises out of focus image information and the detector array is controlled so as to obtain such information during a period in which the time delay integration sensor does not capture first image information.

23. The image scanning apparatus of claim 21, wherein the detector array is controlled so as to obtain third image information comprising focused image information during a period in which the time delay integration sensor obtains first image information.

24. The image scanning apparatus of claim 23, wherein the third image information is also used together with the second image information to calculate a focus position for obtaining the first image information.

16. A method of focusing an image scanning apparatus comprising:

capturing first image information of a target using a time delay integration sensor, the first image information corresponding to a first portion of light received from the target that is used to generate an image of the target;

capturing second image information of the target using a detector array, the second image information corresponding to a second portion of light received from the target;

calculating a focus position for use in capturing the first image information using the second image information; and

adjusting the focus of the image scanning apparatus according to the calculated focus position;

generating relative motion between the time delay integration sensor and the target before capturing further first image information at the calculated focus position, the further first image information corresponding to light received from a further part of the target.

17. The method of claim 16, wherein the first and second portions are separated in at least one of the following ways:

spatially or chromatically.

18. The method of claim 16, wherein capturing first image information further comprises:

irradiating the target to enable the target to fluoresce; and

the first image information comprises image information corresponding to the fluorescence of the target.

19. The method of claim 16, wherein the capture of second image information occurs before the capture of first image information and so the first and second portions of light received from the target are temporally separated.

20. The method of claim 19, wherein the capture of second image information comprises performing a focus scan of the target before capturing first image information.

22. The method of claim 16, wherein the second image information comprises out of focus image information and such information is captured during a period in which the time delay integration sensor does not capture first image information.

25. The method of claim 22, further comprising capturing third image information of the target using the detector array comprising focused image information during a period in which the time delay integration sensor captures first image information.

26. The method of claim 25, wherein the third image information is used together with the second image information to calculate a focus position for obtaining the first image information.