MICRORNAS DIFFERENTIALLY EXPRESSED IN CERVICAL CANCER AND USES THEREOF

• US 20090186348A1
• Filed: 09/12/2008
• Published: 07/23/2009
• Est. Priority Date: 09/14/2007
• Status: Active Grant

• Current Assignee: Asuragen, Inc.
• Original Assignee: Asuragen, Inc.
• Inventors: Elizondo, Laura, Huibregtse, Sylvie Beaudenon, Labourier, Emmanuel
• Granted Patent: US 8,361,714 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/6886 for cancer immunoassay for cancer G01N33/574
• CPC Class Codes: C12Q 2600/106 Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
• CPC Class Codes: C12Q 2600/112 Disease subtyping, staging or classification
• CPC Class Codes: C12Q 2600/178 miRNA, siRNA or ncRNA

Abstract

The present invention concerns methods and compositions for identifying a miRNA profile for a particular condition, such as cervical disease, and using the profile in assessing the condition of a patient.

Claims

1. A method for diagnosing a condition in a patient comprising measuring an expression profile of one or more miRNAs in a sample from a patient suspected of having a pre-cancerous or cancerous cervical condition, wherein a difference in the expression profile in the sample from the patient and an expression profile of a normal sample or a reference is indicative of a pre-cancerous or cancerous condition;

wherein one or more miRNA is selected from hsa-let-7a, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f, hsa-let-7g, hsa-let-7i, hsa-miR-1, hsa-miR-10a, hsa-miR-10b, hsa-miR-100, hsa-miR-101, hsa-miR-106a, hsa-miR-106b, hsa-miR-124a, hsa-miR-125a, hsa-miR-125b, hsa-miR-126, hsa-miR-126-AS, hsa-miR-127, hsa-miR-1300a, hsa-miR-130b, hsa-miR-133a, hsa-miR-134, hsa-miR-135b, hsa-miR-139, hsa-miR-142-5p, hsa-miR-140, hsa-miR-141, hsa-miR-143, hsa-miR-145, hsa-miR-146a, hsa-miR-149, hsa-miR-150, hsa-miR-152, hsa-miR-153, hsa-miR-154, hsa-miR-155, hsa-miR-15b, hsa-miR-16, hsa-miR-17-5p, hsa-miR-18a, hsa-miR-181a, hsa-miR-181b, hsa-miR-182, hsa-miR-183, hsa-miR-185, hsa-miR-186, hsa-miR-187, hsa-miR-189, hsa-miR-190, hsa-miR-195, hsa-miR-196a, hsa-miR-196b, hsa-miR-199a, hsa-miR-199a-AS, hsa-miR-199b, hsa-miR-20a, hsa-miR-200a, hsa-miR-200b, hsa-miR-200c, hsa-miR-203, hsa-miR-204, hsa-miR-205, hsa-miR-21, hsa-miR-210, hsa-miR-214, hsa-miR-215, hsa-miR-218, hsa-miR-223, hsa-miR-224, hsa-miR-23b, hsa-miR-24, hsa-miR-25, hsa-miR-26a, hsa-miR-26b, hsa-miR-27b, hsa-miR-28, hsa-miR-296, hsa-miR-299-5p, hsa-miR-29a, hsa-miR-29b, hsa-miR-29c, hsa-miR-302d, hsa-miR-30a-3p, hsa-miR-30a-5p, hsa-miR-30b, hsa-miR-30d, hsa-miR-31, hsa-miR-320, hsa-miR-324-3p, hsa-miR-325, hsa-miR-328, hsa-miR-330, hsa-miR-335, hsa-miR-339, hsa-miR-361, hsa-miR-365, hsa-miR-368, hsa-miR-370, hsa-miR-373-AS, hsa-miR-376a, hsa-miR-377, hsa-miR-379, hsa-miR-381, hsa-miR-382, hsa-miR-423, hsa-miR-424, hsa-miR-429, hsa-miR-432, hsa-miR-450, hsa-miR-491, hsa-miR-492, hsa-miR-495, hsa-miR-500, hsa-miR-501, hsa-miR-505, hsa-miR-513, hsa-miR-515-5p, hsa-miR-518b, hsa-miR-520d, hsa-miR-92, hsa-miR-93, hsa-miR-95, hsa-miR-98, hsa-miR-99a, hsa-miR-99b, ambi-miR-7029, hsa-miR-498, ambi-miR-7062, ambi-miR-7066, ambi-miR-7068-1, ambi-miR-7070, hsa-miR-497, ambi-miR-7074, ambi-miR-7075, ambi-miR-7079, ambi-miR-7083, ambi-miR-7085, ambi-miR-7100, ambi-miR-7101, hsa-miR-133b, hsa-miR-455, hsa-asg-5021_st1, hsa-asg-13254_st1, hsa-asg-14176_st1, hsa-miR-487b, hsa-miR-411, hsa-miR-574, hsa-miR-542-5p, hsa-asg-5617_st1, hsa-asg-14172_st1, hsa-asg-13304_st2, hsa-asg-13297_st1, hsa-miR157_st2, hsa-cand317_st1, hsa-miR-329, hsa-asg-10202_st2, hsa-miR-369-5p, hsa-asg-13284_st1, hsa-asg-9687_st1, hsa-miR-433, hsa-miR-565, hsa-asg-562_st1, hsa-asg-279_st2, hsa-asg-8411_st2, hsa-asg-7472_st2, hsa-asg-13279_st1, hsa-miR-487a, hsa-cand206_st1, hsa-cand345_st1, hsa-asg-9696_st1, hsa-miR-485-3p, hsa-asg-13166_st2, hsa-miR-594_st2, hsa-miR-299-3p, hsa-asg-924_st1, hsa-miR-539, hsa-asg-10883_st1, hsa-miR-585, hsa-miR-493-5p, hsa-asg-12964_st2, hsa-asg-4557_st2, hsa-asg-10674_st1, hsa-asg-14230_st1, hsa-asg-9681_st1, hsa-miR-628, hsa-asg-13237_st1, hsa-asg-13230_st2, hsa-miR-493-3p, hsa-miR-654, hsa-asg-2919_st1, hsa-asg-8067_st2, hsa-asg-11199_st2, hsa-asg-12346_st2, hsa-asg-11883_st1, hsa-miR102_st2, hsa-asg-2027_st1, hsa-asg-3711_st2, hsa-asg-3145_st1, hsa-asg-7465_st2, hsa-asg-3376_st1, hsa-cand283_st1, hsa-asg-2301_st2, hsa-cand207_st1, hsa-miR-598, hsa-asg-10278_st2, hsa-miR-18b, hsa-asg-11181_st1, hsa-asg-7023_st2, hsa-asg-3597_st2, hsa-asg-5304_st1, hsa-asg-11688_st1, hsa-cand720_st1, hsa-asg-13308_st2, hsa-asg-3038_st2, hsa-asg-13966_st2, hsa-asg-13189_st1, hsa-asg-11938_st1, hsa-asg-5740_st2, hsa-asg-8477_st1, hsa-asg-12325_st2, hsa-asg-12356_st1, hsa-asg-6758_st1, hsa-asg-522_st1, hsa-asg-4564_st2, hsa-asg-6951_st2, hsa-asg-9920_st1, hsa-asg-13613_st2, hsa-miR-184, hsa-miR-503, hsa-miR-485-5p, hsa-miR-494, hsa-miR-504, hsa-miR-211, hsa-miR-99b, hsa-miR-499, hsa-miR-422b, hsa-miR-338, hsa-miR-422a, hsa-miR-331, hsa-miR-489, hsa-miR-324-5p, hsa-miR-151, hsa-miR-17-3p, hsa-miR-340, hsa-miR-194, hsa-let-7d*, hsa-let-7e*, hsa-miR-100*, hsa-miR-101*, hsa-miR-106b*, hsa-miR-10b*, hsa-miR-125a-5p, hsa-miR-125b-2*, hsa-miR-126*, hsa-miR-127-3p, hsa-miR-129-3p, hsa-miR-135a, hsa-miR-136, hsa-miR-136*, hsa-miR-139-5p, hsa-miR-140-3p, hsa-miR-140-5p, hsa-miR-141*, hsa-miR-142-3p, hsa-miR-143*, hsa-miR-144, hsa-miR-144*, hsa-miR-145*, hsa-miR-146b-5p, hsa-miR-151-3p, hsa-miR-154*, hsa-miR-15b*, hsa-miR-17, hsa-miR-181c, hsa-miR-181d, hsa-miR-183*, hsa-miR-193a-5p, hsa-miR-195*, hsa-miR-197, hsa-miR-199a-5p, hsa-miR-199b-3p, hsa-miR-199b-5p, hsa-miR-20a*, hsa-miR-21*, hsa-miR-212, hsa-miR-214*, hsa-miR-24-1*, hsa-miR-26b*, hsa-miR-28-3p, hsa-miR-28-5p, hsa-miR-29a*, hsa-miR-29b-1*, hsa-miR-29b-2*, hsa-miR-29c*, hsa-miR-30a, hsa-miR-30a*, hsa-miR-30b*, hsa-miR-30c-2*, hsa-miR-31*, hsa-miR-32, hsa-miR-330-3p, hsa-miR-337-3p, hsa-miR-337-5p, hsa-miR-345, hsa-miR-361-5p, hsa-miR-374a, hsa-miR-374b, hsa-miR-374b*, hsa-miR-375, hsa-miR-376b, hsa-miR-376c, hsa-miR-377*, hsa-miR-410, hsa-miR-424*, hsa-miR-425, hsa-miR-431*, hsa-miR-450a, hsa-miR-451, hsa-miR-455-3p, hsa-miR-455-5p, hsa-miR-483-3p, hsa-miR-486-5p, hsa-miR-488, hsa-miR-490-3p, hsa-miR-499-5p, hsa-miR-501-5p, hsa-miR-505*, hsa-miR-512-3p, hsa-miR-513c, hsa-miR-517*, hsa-miR-542-3p, hsa-miR-543, hsa-miR-574-3p, hsa-miR-602, hsa-miR-628-5p, hsa-miR-629*, hsa-miR-630, hsa-miR-650, hsa-miR-654-3p, hsa-miR-654-5p, hsa-miR-655, hsa-miR-656, hsa-miR-744, hsa-miR-766, hsa-miR-768-3p, hsa-miR-873, hsa-miR-885-5p, hsa-miR-886-3p, hsa-miR-886-5p, hsa-miR-889, hsa-miR-93*, hsa-miR-940, hsa-miR-944, hsa-miR-96, hsa-miR-99a*, or hsa-miR-99b*.

2. The method of claim 1, wherein the miRNA is one or more of hsa-miR-1, hsa-miR-100, hsa-miR-133a, hsa-miR-134, hsa-miR-143, hsa-miR-154, hsa-miR-182, hsa-miR-183, hsa-miR-195, hsa miR-204, hsa-miR-205, hsa-miR-21, hsa-miR-218, hsa-miR-224, hsa-miR-299-5p, hsa-miR-31, hsa-miR-368, hsa-miR-376a, hsa-miR-424, hsa-miR-99a, ambi-miR-7029, hsa-miR-497, ambi-miR-7101, hsa-miR-133b, hsa-miR-455, hsa-asg-5021_st1, hsa-asg-13254_st1, hsa-asg-14176_st1, hsa-miR-487b, hsa-miR-411, hsa-miR-574, hsa-miR-542-5p, hsa-asg-5617_st1, hsa-asg-14172_st1, hsa-asg-13304_st2, hsa-asg-13297_st1, hsa-miR157_st2, hsa-cand317_st1, hsa-miR-329, hsa-asg-10202_st2, hsa-miR-369-5p, hsa-asg-13284_st1, hsa-asg-9687_st1, hsa-miR-433, hsa-miR-565, hsa-asg-562_st1, hsa-asg-279_st2, hsa-asg-8411_st2, hsa-asg-7472_st2, hsa-asg-13279_st1, hsa-miR-487a, hsa-cand206_st1, hsa-cand345_st1, hsa-asg-9696_st1, hsa-miR-485-3p, hsa-asg-13166_st2, hsa-miR-594_st2, hsa-miR-299-3p, hsa-asg-924_st1, hsa-miR-539, hsa-asg-10883_st1, hsa-miR-184, hsa-miR-503, hsa-miR-485-5p, hsa-miR-494, hsa-miR-504, hsa-miR-211, hsa-miR-99b, hsa-miR-495, hsa-miR-381, hsa-miR-127, hsa-miR-124a, hsa-miR-432, hsa-mir-214, hsa-miR-379, hsa-miR-189, hsa-miR-130a, hsa-miR-382, hsa-miR-885-5p, hsa-miR-145*, hsa-miR-517*, hsa-miR-139-5p, hsa-miR-145, hsa-miR-376c, hsa-miR-135b, hsa-miR-136*, hsa-miR-99a*, hsa-miR-125b-2*, hsa-miR-31*, hsa-miR-125b, hsa-miR-96, hsa-miR-451, hsa-miR-143*, hsa-miR-135a, hsa-miR-141, hsa-miR-375, hsa-miR-21*, hsa-miR-654-3p, hsa-miR-455-5p, hsa-miR-337-3p, hsa-miR-886-5p, hsa-miR-410, hsa-miR-944, or hsa-miR-337-5p.

3. The method of claim 2, wherein the miRNA is hsa-miR-1, hsa-miR-21 or both hsa-miR-1 and hsa-miR-21.

4. The method of claim 1, wherein differential expression of one or more miRNA is indicative of cervical carcinoma, wherein the miRNA is one or more of hsa-let-7a, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f, hsa-let-7g, hsa-let-7i, hsa-miR-1, hsa-miR-10a, hsa-miR-10b, hsa-miR-100, hsa-miR-101, hsa-miR-106a, hsa-miR-106b, hsa-miR-124a, hsa-miR-125a, hsa-miR-125b, hsa-miR-126, hsa-miR-126-AS, hsa-miR-127, hsa-miR-130a, hsa-miR-130b, hsa-miR-133a, hsa-miR-134, hsa-miR-135b, hsa-miR-139, hsa-miR-140, hsa-miR-141, hsa-miR-143, hsa-miR-145, hsa-miR-146a, hsa-miR-149, hsa-miR-150, hsa-miR-152, hsa-miR-153, hsa-miR-154, hsa-miR-155, hsa-miR-15b, hsa-miR-16, hsa-miR-17-5p, hsa-miR-18a, hsa-miR-181a, hsa-miR-181b, hsa-miR-182, hsa-miR-183, hsa-miR-185, hsa-miR-186, hsa-miR-187, hsa-miR-189, hsa-miR-190, hsa-miR-195, hsa-miR-196b, hsa-miR-199a, hsa-miR-199a-AS, hsa-miR-199b, hsa-miR-20a, hsa-miR-200a, hsa-miR-200b, hsa-miR-200c, hsa-miR-203, hsa-miR-204, hsa-miR-205, hsa-miR-21, hsa-miR-210, hsa-miR-214, hsa-miR-215, hsa-miR-218, hsa-miR-223, hsa-miR-224, hsa-miR-23b, hsa-miR-24, hsa-miR-25, hsa-miR-26a, hsa-miR-26b, hsa-miR-27b, hsa-miR-28, hsa-miR-296, hsa-miR-299-5p, hsa-miR-29a, hsa-miR-29b, hsa-miR-29c, hsa-miR-302d, hsa-miR-30a-3p, hsa-miR-30a-5p, hsa-miR-30b, hsa-miR-30d, hsa-miR-31, hsa-miR-320, hsa-miR-324-3p, hsa-miR-328, hsa-miR-330, hsa-miR-335, hsa-miR-339, hsa-miR-361, hsa-miR-365, hsa-miR-368, hsa-miR-370, hsa-miR-373-AS, hsa-miR-376a, hsa-miR-377, hsa-miR-379, hsa-miR-381, hsa-miR-382, hsa-miR-423, hsa-miR-424, hsa-miR-429, hsa-miR-432, hsa-miR-450, hsa-miR-491, hsa-miR-492, hsa-miR-495, hsa-miR-501, hsa-miR-505, hsa-miR-515-5p, hsa-miR-518b, hsa-miR-520d, hsa-miR-92, hsa-miR-93, hsa-miR-95, hsa-miR-98, hsa-miR-99a, hsa-miR-99b, ambi-miR-7029, hsa-miR-498, ambi-miR-7062, ambi-miR-7066, ambi-miR-7068-1, ambi-miR-7070, hsa-miR-497, ambi-miR-7074, ambi-miR-7075, ambi-miR-7079, ambi-miR-7083, ambi-miR-7085, ambi-miR-7100, ambi-miR-7101, hsa-miR-133b, hsa-miR-455, hsa-asg-5021_st1, hsa-asg-13254_st1, hsa-asg-14176_st1, hsa-miR-487b, hsa-miR-411, hsa-miR-574, hsa-miR-542-5p, hsa-asg-5617_st1, hsa-asg-14172_st1, hsa-asg-13304_st2, hsa-asg-13297_st1, hsa-miR157_st2, hsa-cand317_st1, hsa-miR-329, hsa-asg-10202_st2, hsa-miR-369-5p, hsa-asg-13284_st1, hsa-asg-9687_st1, hsa-miR-433, hsa-miR-565, hsa-asg-562_st1, hsa-asg-279_st2, hsa-asg-8411st2, hsa-asg-7472_st2, hsa-asg-13279_st1, hsa-miR-487a, hsa-cand206_st1, hsa-cand345_st1, hsa-asg-9696_st1, hsa-miR-485-3p, hsa-asg-13166_st2, hsa-miR-594_st2, hsa-miR-299-3p, hsa-asg-924_st1, hsa-miR-539, hsa-asg-10883_st1, hsa-miR-585, hsa-miR-493-5p, hsa-asg-12964_st2, hsa-asg-4557_st2, hsa-asg-10674_st1, hsa-asg-14230_st1, hsa-asg-9681_st1, hsa-miR-628, hsa-asg-13237_st1, hsa-asg-13230_st2, hsa-miR-493-3p, hsa-miR-654, hsa-asg-2919_st1, hsa-asg-8067_st2, hsa-asg-11199_st2, hsa-asg-12346_st2, hsa-asg-11883_st1, hsa-miR102_st2, hsa-asg-2027_st1, hsa-asg-3711_st2, hsa-asg-3145_st1, hsa-asg-7465_st2, hsa-asg-3376_st1, hsa-cand283_st1, hsa-asg-2301_st2, hsa-cand207_st1, hsa-miR-598, hsa-asg-10278_st2, hsa-miR-18b, hsa-asg-11181_st1, hsa-asg-7023_st2, hsa-asg-3597_st2, hsa-asg-5304_st1, hsa-asg-11688_st1, hsa-cand720_st1, hsa-asg-13308_st2, hsa-asg-3038_st2, hsa-asg-13966_st2, hsa-asg-13189_st1, hsa-asg-11938_st1, hsa-asg-5740_st2, hsa-asg-8477_st1, hsa-asg-12325_st2, hsa-asg-12356_st1, hsa-asg-6758_st1, hsa-asg-522_st1, hsa-asg-4564_st2, hsa-asg-6951_st2, hsa-asg-9920_st1, hsa-asg-13613_st2, hsa-miR-184, hsa-miR-503, hsa-miR-485-5p, hsa-miR-494, hsa-miR-504, hsa-miR-211, hsa-miR-99b, hsa-miR-499, hsa-miR-422b, hsa-miR-338, hsa-miR-422a, hsa-miR-331, hsa-miR-489, hsa-miR-324-5p, hsa-miR-151, hsa-miR-17-3p, hsa-miR-340, hsa-miR-194, hsa-let-7d*, hsa-let-7e*, hsa-miR-100*, hsa-miR-101*, hsa-miR-106b*, hsa-miR-10b*, hsa-miR-125a-5p, hsa-miR-125b-2*, hsa-miR-126*, hsa-miR-127-3p, hsa-miR-129-3p, hsa-miR-135a, hsa-miR-136, hsa-miR-136*, hsa-miR-139-5p, hsa-miR-140-3p, hsa-miR-140-5p, hsa-miR-141*, hsa-miR-142-3p, hsa-miR-142-5p, hsa-miR-143*, hsa-miR-144, hsa-miR-144*, hsa-miR-145*, hsa-miR-146b-5p, hsa-miR-151-3p, hsa-miR-154*, hsa-miR-15b*, hsa-miR-17, hsa-miR-181c, hsa-miR-181d, hsa-miR-183*, hsa-miR-193a-5p, hsa-miR-195*, hsa-miR-197, hsa-miR-199a-5p, hsa-miR-199b-3p, hsa-miR-199b-5p, hsa-miR-20a*, hsa-miR-21*, hsa-miR-212, hsa-miR-214*, hsa-miR-24-1*, hsa-miR-26b*, hsa-miR-28-3p, hsa-miR-28-5p, hsa-miR-29a*, hsa-miR-29b-1*, hsa-miR-29b-2*, hsa-miR-29c*, hsa-miR-30a, hsa-miR-30a*, hsa-miR-30b*, hsa-miR-30c-2*, hsa-miR-31*, hsa-miR-32, hsa-miR-330-3p, hsa-miR-337-3p, hsa-miR-337-5p, hsa-miR-345, hsa-miR-361-5p, hsa-miR-374a, hsa-miR-374b, hsa-miR-374b*, hsa-miR-375, hsa-miR-376b, hsa-miR-376c, hsa-miR-377*, hsa-miR-410, hsa-miR-424*, hsa-miR-425, hsa-miR-431*, hsa-miR-450a, hsa-miR-451, hsa-miR-455-3p, hsa-miR-455-5p, hsa-miR-483-3p, hsa-miR-486-5p, hsa-miR-488, hsa-miR-490-3p, hsa-miR-499-5p, hsa-miR-501-5p, hsa-miR-505*, hsa-miR-512-3p, hsa-miR-513c, hsa-miR-517*, hsa-miR-542-3p, hsa-miR-543, hsa-miR-574-3p, hsa-miR-602, hsa-miR-628-5p, hsa-miR-629*, hsa-miR-630, hsa-miR-650, hsa-miR-654-3p, hsa-miR-654-5p, hsa-miR-655, hsa-miR-656, hsa-miR-744, hsa-miR-766, hsa-miR-768-3p, hsa-miR-873, hsa-miR-885-5p, hsa-miR-886-3p, hsa-miR-886-5p, hsa-miR-889, hsa-miR-93*, hsa-miR-940, hsa-miR-944, hsa-miR-96, hsa-miR-99a*, or hsa-miR-99b*.

5. The method of claim 1, wherein differential expression of one or more miRNA is indicative of cervical intraepithelial neoplasia or cervical squamous intraepithelial lesions, wherein the miRNA is one or more of hsa-miR-1, hsa-miR-133a, hsa-miR-124a, hsa-miR-187, hsa-miR-204, hsa-miR-145, hsa-miR-143, hsa-miR-325, hsa-miR-500, hsa-miR-196a, hsa-miR-125a, hsa-miR-376a, hsa-miR-505, hsa-miR-100, hsa-miR-99a, hsa-miR-141, hsa-miR-200a, ambi-miR-7029, hsa-miR-223, hsa-miR-205, hsa-miR-146a, hsa-miR-429, hsa-miR-200b, hsa-miR-182, hsa-miR-142-5p, hsa-miR-203, hsa-miR-21, hsa-miR-31, or hsa-miR-513.

6. The method of claim 1, wherein reduced expression of an miRNA in a sample relative to normal or normal adjacent tissue is indicative of a cancerous condition, wherein the miRNA is one or more of hsa-let-7a, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f, hsa-let-7g, hsa-let-7i, hsa-miR-1, hsa-miR-10a, hsa-miR-10b, hsa-miR-100, hsa-miR-101, hsa-miR-124a, hsa-miR-125a, hsa-miR-125b, hsa-miR-126, hsa-miR-126-AS, hsa-miR-127, hsa-miR-130a, hsa-miR-133a, hsa-miR-134, hsa-miR-139, hsa-miR-142-5p, hsa-miR-140, hsa-miR-143, hsa-miR-145, hsa-miR-149, hsa-miR-152, hsa-miR-154, hsa-miR-186, hsa-miR-189, hsa-miR-190, hsa-miR-195, hsa-miR-196a, hsa-miR-196b, hsa-miR-199a, hsa-miR-199a-AS, hsa-miR-199b, hsa-miR-204, hsa-miR-214, hsa-miR-215, hsa-miR-218, hsa-miR-23b, hsa-miR-24, hsa-miR-26a, hsa-miR-26b, hsa-miR-27b, hsa-miR-28, hsa-miR-299-5p, hsa-miR-29a, hsa-miR-29b, hsa-miR-29c, hsa-miR-30a-3p, hsa-miR-30a-5p, hsa-miR-30b, hsa-miR-30d, hsa-miR-320, hsa-miR-324-3p, hsa-miR-325, hsa-miR-328, hsa-miR-335, hsa-miR-361, hsa-miR-365, hsa-miR-368, hsa-miR-376a, hsa-miR-377, hsa-miR-379, hsa-miR-381, hsa-miR-382, hsa-miR-424, hsa-miR-432, hsa-miR-450, hsa-miR-495, hsa-miR-500, hsa-miR-505, hsa-miR-513, hsa-miR-98, hsa-miR-99a, hsa-miR-99b, ambi-miR-7029, ambi-miR-7066, ambi-miR-7068-1, ambi-miR-7070, hsa-miR-497, ambi-miR-7075, ambi-miR-7083, ambi-miR-7085, ambi-miR-7100, ambi-miR-7101, hsa-miR-133b, hsa-miR-455, hsa-asg-5021_st1, hsa-asg-13254_st1, hsa-asg-14176_st1, hsa-miR-487b, hsa-miR-411, hsa-miR-574, hsa-miR-542-5p, hsa-asg-5617_st1, hsa-asg-14172_st1, hsa-asg-13304_st2, hsa-asg-13297_st1, hsa-miR157_st2, hsa-miR-329, hsa-asg-10202_st2, hsa-miR-369-5p, hsa-asg-13284_st1, hsa-asg-9687_st1, hsa-miR-433, hsa-miR-565, hsa-asg-562_st1, hsa-asg-279_st2, hsa-asg-8411_st2, hsa-asg-7472_st2, hsa-asg-13279_st1, hsa-miR-487a, hsa-cand206_st1, hsa-cand345_st1, hsa-asg-9696_st1, hsa-miR-485-3p, hsa-asg-13166_st2, hsa-miR-594_st2, hsa-miR-299-3p, hsa-miR-539, hsa-asg-10883_st1, hsa-miR-585, hsa-miR-493-5p, hsa-asg-12964_st2, hsa-asg-4557_st2, hsa-asg-10674_st1, hsa-asg-14230_st1, hsa-asg-9681_st1, hsa-miR-628, hsa-asg-13237_st1, hsa-asg-13230_st2, hsa-miR-493-3p, hsa-miR-654, hsa-asg-33_st1, hsa-asg-8067_st2, hsa-asg-11199_st2, hsa-asg-12346_st2, hsa-asg-11883_st1, hsa-miR102_st2, hsa-asg-2027_st1, hsa-asg-3145_st1, hsa-asg-7465_st2, hsa-asg-3376_st1, hsa-cand283_st1, hsa-asg-2301_st2, hsa-cand207_st1, hsa-miR-598, hsa-asg-10278_st2, hsa-asg-11181_st1, hsa-asg-7023_st2, hsa-asg-3597_st2, hsa-asg-5304_st1, hsa-cand720_st1, hsa-asg-13308_st2, hsa-asg-3038_st2, hsa-asg-13966_st2, hsa-asg-13189_st1, hsa-asg-11938_st1, hsa-asg-5740_st2, hsa-asg-8477_st1, hsa-asg-12325_st2, hsa-asg-12356_st1, hsa-asg-6758_st1, hsa-asg-522_st1, hsa-asg-4564_st2, hsa-asg-6951_st2, hsa-asg-9920_st1, hsa-asg-13613_st2, hsa-miR-184, hsa-miR-503, hsa-miR-485-5p, hsa-miR-494, hsa-miR-504, hsa-miR-211, hsa-miR-99b, hsa-miR-499, hsa-miR-422b, hsa-miR-338, hsa-miR-422a, hsa-miR-331, hsa-miR-489, hsa-miR-324-5p, hsa-miR-151, hsa-miR-17-3p, hsa-miR-340, hsa-miR-194, hsa-miR-885-5p, hsa-miR-145*, hsa-miR-517*, hsa-miR-139-5p, hsa-miR-376c, hsa-miR-136*, hsa-miR-99a*, hsa-miR-125b-2*, hsa-miR-451, hsa-miR-143*, hsa-miR-135a, hsa-miR-375, hsa-miR-654-3p, hsa-miR-455-5p, hsa-miR-337-3p, hsa-miR-886-5p, hsa-miR-410, hsa-miR-337-5p, or a combination thereof.

7. The method of claim 1, wherein increased expression of an miRNA in a sample relative to normal or normal adjacent tissue is indicative of a cancerous condition, wherein the miRNA is one or more of hsa-miR-106a, hsa-miR-106b, hsa-miR-130b, hsa-miR-135b, hsa-miR-205, hsa-miR-141, hsa-miR-182, hsa-miR-146a, hsa-miR-150, hsa-miR-155, hsa-miR-15b, hsa-miR-16, hsa-miR-17-5p, hsa-miR-18a, hsa-miR-181a, hsa-miR-181b, hsa-miR-183, hsa-miR-185, hsa-miR-20a, hsa-miR-200a, hsa-miR-200b, hsa-miR-200c, hsa-miR-203, hsa-miR-21, hsa-miR-210, hsa-miR-223, hsa-miR-224, hsa-miR-25, hsa-miR-31, hsa-miR-330, hsa-miR-339, hsa-miR-423, hsa-miR-429, hsa-miR-492, hsa-miR-92, hsa-miR-93, hsa-miR-518b, hsa-miR-520d, hsa-miR-491, hsa-miR-501, hsa-miR-515-5p, ambi-miR-7062, ambi-miR-7074, ambi-miR-7079, hsa-asg-11688_st1, hsa-miR-18b, hsa-miR-18a, hsa-asg-3711_st2, hsa-asg-2919_st1, hsa-asg-924_st1, hsa-cand317_st1, hsa-miR-31*, hsa-miR-96, hsa-miR-21*, hsa-miR-944, or a combination thereof.

8. The method of claim 1, wherein reduced expression of an miRNA in a sample relative to normal or normal adjacent tissue is indicative of a pre-cancerous condition, wherein the miRNA is one or more of hsa-miR-1, hsa-miR-133a, hsa-miR-124a, hsa-miR-187, hsa-miR-204, hsa-miR-143, hsa-miR-145, hsa-miR-325, hsa-miR-99a, hsa-miR-100, hsa-miR-376a, hsa-miR-505, hsa-miR-125a, hsa-miR-196a, hsa-miR-500 or a combination thereof.

9. The method of claim 1, wherein increased expression of an miRNA in a sample relative to normal or normal adjacent tissue is indicative of a pre-cancerous condition, wherein the miRNA is one or more of hsa-miR-205, hsa-miR-182, hsa-miR-141, hsa-miR-142-5p, hsa-miR200a, hsa-miR-200b, hsa-miR-223, hsa-miR-21, hsa-miR-203, hsa-miR-31, ambi-miR-7029, hsa-miR-146a, hsa-miR-429, hsa-miR-513 or a combination thereof.

10. The method of claim 1, wherein the sample is isolated RNA, fresh tissue or cells, frozen tissue or cells, fixed tissue or cells, or embedded tissue or cells.

11. The method of claim 1, wherein the sample from the patient and the normal sample are cervix samples.

12. The method of claim 1, wherein the pre-cancerous condition is cervical squamous intraepithelial lesion or cervical intraepithelial neoplasia.

13. The method of claim 1, wherein the cancerous condition is cervical squamous cell carcinoma.

14. The method of claim 1, further comprising obtaining a sample from the patient.

15. The method of claim 14, further comprising labeling miRNA from the sample.

16. The method of claim 15, further comprising hybridizing the labeled miRNA to one or more miRNA probes.

21. The method of claim 1, wherein expression of the miRNA is determined by an amplification assay or a hybridization assay.

22. The method of claim 21, wherein the miRNA amplified is all or part of one or more of hsa-miR-1, hsa-miR-15b, hsa-miR-133a, hsa-miR-143, hsa-miR-205, hsa-miR-21, hsa-miR-204, hsa-miR-195, hsa-miR-100, hsa-miR-99a, hsa-miR-368, hsa-miR-183 or combination thereof.

17-20. -20. (canceled)

23-27. -27. (canceled)

28. A method of detecting a cervical cell comprising evaluating the expression of an miRNA selected from one or more of hsa-let-7a, hsa-let-7b, hsa-let-7c, hsa-let-7i, hsa-miR-100, hsa-miR-101, hsa-miR-106a, hsa-miR-125a, hsa-miR-125b, hsa-miR-126-AS, hsa-miR-127, hsa-miR-130a, hsa-miR-134, hsa-miR-142-3p, hsa-miR-143, hsa-miR-145, hsa-miR-148b, hsa-miR-149, hsa-miR-151, hsa-miR-152, hsa-miR-154, hsa-miR-15a, hsa-miR-15b, hsa-miR-16, hsa-miR-17-5p, hsa-miR-181a, hsa-miR-185, hsa-miR-186, hsa-miR-187, hsa-miR-192, hsa-miR-193a, hsa-miR-195, hsa-miR-196a, hsa-miR-196b, hsa-miR-199a, hsa-miR-199a-AS, hsa-miR-199b, hsa-miR-19a, hsa-miR-20a, hsa-miR-203, hsa-miR-205, hsa-miR-21, hsa-miR-214, hsa-miR-215, hsa-miR-22, hsa-miR-221, hsa-miR-223, hsa-miR-23b, hsa-miR-24, hsa-miR-25, hsa-miR-26a, hsa-miR-26b, hsa-miR-299-5p, hsa-miR-29b, hsa-miR-29c, hsa-miR-302c-AS, hsa-miR-30a-3p, hsa-miR-30a-5p, hsa-miR-30b, hsa-miR-30c, hsa-miR-30e-3p, hsa-miR-30e-5p, hsa-miR-31, hsa-miR-320, hsa-miR-324-3p, hsa-miR-335, hsa-miR-338, hsa-miR-342, hsa-miR-34a, hsa-miR-361, hsa-miR-365, hsa-miR-367, hsa-miR-368, hsa-miR-370, hsa-miR-374, hsa-miR-376a, hsa-miR-379, hsa-miR-381, hsa-miR-423, hsa-miR-424, hsa-miR-450, hsa-miR-7, hsa-miR-93, hsa-miR-95, hsa-miR-96, hsa-miR-99a, hsa-miR-99b, ambi-miR-7027, ambi-miR-7029, hsa-miR-509, hsa-miR-193b, ambi-miR-7039, hsa-miR-526b, hsa-miR-498, hsa-miR-452, ambi-miR-7062, ambi-miR-7070, hsa-miR-497, ambi-miR-7079, ambi-miR-7083, ambi-miR-7085, hsa-miR-503, or ambi-miR-7101 or complements thereof.

29. A kit for analysis of a pathological sample by assessing miRNA profile for a sample comprising, in suitable container means, two or more miRNA hybridization or amplification reagents comprising one or more of the miRNA listed in Table 1.

30-38. -38. (canceled)