Pathway Analysis of Cell Culture Phenotypes and Uses Thereof
First Claim
1. An engineered cell line characterized by an improved cell culture phenotype as compared to the corresponding wild type or parental cell line comprising a population of engineered cells, each of which comprising an engineered construct up-regulating or down-regulating one or more genes or proteins selected from Tables 1-35, wherein said up-regulating or down-regulating one or more genes or proteins confers the improved cell culture phenotype.
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Abstract
The present invention provides methods for systematically identifying genes, proteins and/or related pathways that regulate or indicative of cell phenotypes. The present invention further provides methods for manipulating the identified genes, proteins and/or pathways to engineer improved cell lines and/or to evaluate or select cell lines with desirable phenotypes.
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Citations
41 Claims
- 1. An engineered cell line characterized by an improved cell culture phenotype as compared to the corresponding wild type or parental cell line comprising a population of engineered cells, each of which comprising an engineered construct up-regulating or down-regulating one or more genes or proteins selected from Tables 1-35, wherein said up-regulating or down-regulating one or more genes or proteins confers the improved cell culture phenotype.
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10-13. -13. (canceled)
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15. (canceled)
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17. A method of improving a cell line, the method comprising modifying one or more pathways selected from
FIGS. 1-31 .
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25-28. -28. (canceled)
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31. (canceled)
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33. A method of evaluating a cell culture phenotype of a cell line, the method comprising:
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detecting, in a sample of cultured cells, an expression level of at least one protein or gene selected from Tables 1-35; comparing the expression level to a reference level; wherein the comparison is indicative of the cell culture phenotype.
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34-40. -40. (canceled)
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41. A method of evaluating a cell culture phenotype of a cell line, the method comprising:
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determining, in a sample of cultured cells, a signaling strength of at least one pathway selected from FIGS. 1-31 ;comparing the signaling strength to a reference; wherein the comparison is indicative of the cell culture phenotype.
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Specification