Biochip cartridge

• US 20090186403A1
• Filed: 08/05/2008
• Published: 07/23/2009
• Est. Priority Date: 01/09/2003
• Status: Abandoned Application

• Current Assignee: Yokogawa Electric Corporation
• Original Assignee: Yokogawa Electric Corporation
• Inventors: Tanaami, Takeo, Fukushima, Kazuhisa
• Application Number:
  US12/222,203
• Publication Number:
  US 20090186403A1
• Time in Patent Office: Days
• US Class Current: 435/287.200
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0822 Slides
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0683 mechanically breaking a wall or membrane within a channel or chamber
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 3/505 flexible containers not provided for above
• CPC Class Codes: G01N 2035/00158 Elements containing microarrays, i.e. "biochip"
• CPC Class Codes: G01N 21/11 Filling or emptying of cuvettes
• CPC Class Codes: G01N 21/64 Fluorescence; Phosphorescence
• CPC Class Codes: G01N 33/54386 Analytical elements

Abstract

The present invention provides a biochip cartridge wherein an elastic body is used for a substrate member in order to stabilize the feeding of blood or solution and whereby it is possible to avoid the risk of accidental contact of the operator with solutions due to mishandling.

The biochip cartridge comprises a tabular substrate member formed using an elastic material and a flexible cover airtightly attached to the surface of the substrate member, wherein at least an area for storing biopolymers, an area for detecting desired biopolymers from the biopolymers that have been preprocessed, and a flow path for connecting the areas is formed on the substrate member, so that biopolymers can be successively moved from the biopolymer storage area to the biopolymer detection area through the flow path.

Claims

1. A biochip cartridge comprising:

a tabular substrate member formed using an elastic material; and

a flexible cover airtightly attached to the surface of said substrate member,

wherein at least a collection area for storing biopolymers, a preprocessing area for applying preprocessing to said biopolymers, a detection area for detecting biopolymers from said preprocessed biopolymers and gaps serving as a flow path for connecting said collection area, said preprocessing area and said detection area are formed in said substrate member, so that biopolymers can be successively transferred from said collection area through said preprocessing area to said detection area,

wherein said biopolymers are transferred by pressing said cover with a roller-like rigid body and squeezing each gap formed in said substrate member from said collection area through said preprocessing area toward said detection area; and

wherein said biochip cartridge is made separable into a first housing for extracting and storing said biopolymers from a biological sample and a second housing having a joint for attachably and detachably coupling with said first housing to enable biopolymers to be injected from said first housing, so that biological samples can be injected into said first housing and transferred from said first housing said to second housing at different timings.

2. The biochip cartridge of claim 1, wherein said biopolymers are DNA, RNA such as mRNA or cDNA, or protein.

3. The biochip cartridge of claim 1, wherein said second housing is provided with a substrate onto which second biopolymers having sequences complementary to said biopolymers are fixed so that said second biopolymers are hybridized with biopolymers injected from said first housing.

4. The biochip cartridge of claim 1, wherein at least said first housing is formed using a material having good flexibility.

5. The biochip cartridge of claim 3, wherein said second housing is formed using a transparent material.