Cholesterol Sensor
First Claim
1. A method for the determination of the amount of cholesterol in lipoproteins other than high density lipoproteins in a lipoprotein-containing sample, said method comprising (a) electrochemically determining the amount of cholesterol bound to high density lipoproteins in the sample, (b) electrochemically determining the total amount of cholesterol in the sample, and subtracting the result of (a) from the result of (b).
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Abstract
A method for the determination of the amount of cholesterol in lipoproteins other than high density lipoproteins in a lipoprotein-containing sample, said method comprising (a) electrochemically determining the amount of cholesterol bound to high density lipoproteins in the sample, (b) electrochemically determining the total amount of cholesterol in the sample, and subtracting the result of (a) from the result of (b).
47 Citations
18 Claims
- 1. A method for the determination of the amount of cholesterol in lipoproteins other than high density lipoproteins in a lipoprotein-containing sample, said method comprising (a) electrochemically determining the amount of cholesterol bound to high density lipoproteins in the sample, (b) electrochemically determining the total amount of cholesterol in the sample, and subtracting the result of (a) from the result of (b).
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13. A kit for the determination of the amount of cholesterol in lipoproteins other than high density lipoproteins in a lipoprotein-containing sample, the kit comprising
a first electrochemical cell having a working electrode, a reference or pseudo reference electrode and optionally a separate counter electrode; -
a first series of reagents comprising (a1) a cholesterol ester hydrolysing reagent; (a2) cholesterol dehydrogenase; (a3) a coenzyme; (a4) a redox agent capable of being oxidised or reduced to form a product; and (a5) a surfactant which selectively breaks down high density lipoproteins, said first series of reagents being associated with said first electrochemical cell; a second electrochemical cell having a working electrode, a reference or pseudo-reference electrode and optionally a separate counter electrode; a second series of reagents comprising (b1) a cholesterol ester hydrolysing reagent; (b2) cholesterol dehydrogenase; (b3) a coenzyme; (b4) a redox agent capable of being oxidised or reduced to form a product; and
optionally(b5) a surfactant, said second series of reagents being associated with said second electrochemical cell; a power supply for applying a potential across each cell; a measuring instrument for measuring the resulting electrochemical response of each cell; and a calculator for calculating the amount of cholesterol in lipoproteins other than high density lipoproteins. - View Dependent Claims (14, 15)
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16. A method of operating a kit, which kit is for the determination of the amount of cholesterol in lipoproteins other than high density lipoproteins in a lipoprotein-containing sample the kit comprising
a first electrochemical cell having a working electrode, a reference or pseudo reference electrode and optionally a separate counter electrode; -
a first series of reagents comprising (a1) a cholesterol ester hydrolysing reagent; (a2) cholesterol dehydrogenase; (a3) a coenzyme; (a4) a redox agent capable of being oxidised or reduced to form a product; and (a5) a surfactant which selectively breaks down high density lipoproteins, said first series of reagents being associated with said first electrochemical cell; a second electrochemical cell having a working electrode, a reference or pseudo reference electrode and optionally a separate counter electrode; a second series of reagents comprising (b1) a cholesterol ester hydrolysing reagent; (b2) cholesterol dehydrogenase; (b3) a coenzyme; (b4) a redox agent capable of being oxidised or reduced to form a product; and
optionally(b5) a surfactant, said second series of reagents being associated with said second electrochemical cell; a power supply for applying a potential across each cell; a measuring instrument for measuring the resulting electrochemical response of each cell; and a calculator for calculating the amount of cholesterol in lipoproteins other than high density lipoproteins, said method comprising (i) contacting a sample with the first series of reagents, such that the resulting mixture of sample and first series of reagents is in contact with the working electrode of the first electrochemical cell; (ii) contacting the sample with the second series of reagents, such that the resulting mixture of sample and second series of reagents is in contact with the working electrode of the second electrochemical cell; (iii) applying a potential across each electrochemical cell; (iv) electrochemically detecting the amount of product formed in each cell by measuring the resulting electrochemical response; and (v) calculating the amount of cholesterol bound to non-HDL lipoproteins in the sample. - View Dependent Claims (17, 18)
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Specification