Multipotent/pluripotent cells and methods
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Abstract
Described herein are multipotent stem cells, e.g., human and other mammalian pluripotent stem cells, and related methods.
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Citations
195 Claims
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1-132. -132. (canceled)
- 133. A human stem cell that is pluripotent, somatic, non-embryonic, and having the property of long-term self renewal
- 145. A human stem cell that is somatic, non-embryonic, alkaline phosphatase positive, and expresses two or more genes selected from the group consisting of TDGF 1, Dnmt3b, FoxD3, GDF3, Cyp26a1, TERT, zfp42, Sox2, Oct3/4, and Nanog.
- 158. A human stem cell generated by a method comprising forcing the expression of an Oct3/4 polypeptide, a Sox2 polypeptide, and a Klf4 polypeptide in human postnatal cells to obtain one or more colonies of cells that have a high nucleus to cytoplasm ratio and are smaller in size than cells surrounding the one or more colonies, and isolating at least one of the one or more colonies.
- 164. The human stem cell of claim 164, wherein the method does not comprise forcing expression of a c-Myc polypeptide.
- 169. A method for generating a human stem cell, comprising forcing expression of polypeptides in human postnatal cells, wherein the polypeptides comprise two or more of an Oct3/4 polypeptide, a Sox2 polypeptide, a Klf4 polypeptide, and a c-Myc polypeptide.
- 181. A method for generating an autologous stem cell from a human subject, comprising forcing expression of an Oct3/4 polypeptide, a Sox2 polypeptide, and a Klf4 polypeptide in a cultured population of non-embryonic postnatal cells from the human subject.
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188. A method for performing cell transplantation in a subject in need thereof, comprising:
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(i) identifying a donor that is immunocompatible with the subject; (ii) generating an induced pluripotent stem cell line from postnatal cells of the healthy donor; and (iii) transplanting one or more cells differentiated from the induced pluripotent stem cell line into the subject. - View Dependent Claims (189)
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190. A method for identifying an agent that stimulates pluripotency or multipotency in a human somatic cell comprising:
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(i) providing first and second cultured human somatic cells; (ii) contacting the first cultured human somatic cell with a test agent; (iii) contacting the second cultured human somatic cell with a negative control agent; (iv) determining expression levels of an embryonic stem cell marker gene in the contacted first and second cultured cells; and comparing the expression levels determined in step (iii) and indicating that the test agent stimulates pluripotency or multipotency if the embryonic stem cell marker gene expression level in the contacted first cultured cell is greater than that determined in the contacted second cultured cell, and indicating that the test agent fails to stimulate multipotency or pluripotency if the expression level of the embryonic stem cell marker gene in the contacted first cultured cell is the same or less than that determined in the contacted second cultured cell, wherein determining the expression levels of the embryonic stem cell marker gene comprises determining the expression levels of Tert or Cyp26A1. - View Dependent Claims (191, 192, 193)
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194. A composition comprising at least one of:
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(i) a purified polypeptide comprising the amino acid sequence of a protein transduction domain and an Oct3/4 polypeptide; (ii) a carrier reagent and a purified Oct3/4 polypeptide; (iii) a purified polypeptide comprising the amino acid sequence of a protein transduction and a Sox2 polypeptide; (iv) a carrier reagent and a purified Sox2 polypeptide; (v) a purified polypeptide comprising the amino acid sequence of a protein transduction domain and a Klf4 polypeptide; (vi) a carrier reagent and a purified Klf4 polypeptide; (vii) a purified polypeptide comprising the amino acid sequence of a protein transduction domain and a c-Myc polypeptide; (viii) a carrier reagent and a purified c-Myc;
or(ix) any combination of (i) to (viii) - View Dependent Claims (195)
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Specification