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METHOD FOR ASSAYING REG IV mRNA

  • US 20090191562A1
  • Filed: 01/15/2009
  • Published: 07/30/2009
  • Est. Priority Date: 10/28/2005
  • Status: Abandoned Application
First Claim
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1. A method for assaying Regenerating islet-derived family, member 4 (regenerating gene type 4) mRNA (Reg IV mRNA) present in a sample, the method comprising:

  • (1) using a first primer homologous to at least a portion downstream from the 5′

    end of a specified nucleotide sequence of said mRNA and a second primer complementary to at least a portion upstream from the 3′

    end of said specified nucleotide sequence, to produce double-stranded DNA comprising a promoter sequence and said specified nucleotide sequence downstream from said promoter sequence, where at least one of said first and second primers has said promoter sequence at the 5′

    end,(2) using said double-stranded DNA as template to produce an RNA transcript,(3) using said RNA transcript in turn as template for DNA synthesis, to amplify said RNA transcript in a chain-reaction, and(4) assaying the amount of said RNA transcript,wherein the combination of said first and second primers is selected from the group consisting of;

    (a) a combination wherein said first primer consists of the sequence selected from the group consisting of SEQ ID NOs;

    10, 12, 18, and 20, and said second primer consists of the sequence of SEQ ID NO;

    11;

    (b) a combination wherein said first primer consists of the sequence selected from the group consisting of SEQ ID NOs;

    18 and 19, and said second primer consists of the sequence of SEQ ID NO;

    31; and

    (c) a combination of the sequence of SEQ ID NO;

    18 as the first primer and the sequence of SEQ ID NO;

    33 as the second primer.

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