Nucleic Acid Interaction Analysis
First Claim
1. A method of preparing at least one isolated linear composite nucleic acid molecule comprising at least one first tag from at least one first nucleic acid molecule and at least one second tag from at least one second nucleic acid molecule, wherein the first and second nucleic acid molecules interact in a nucleic acid mixture and wherein the first and second tags are from different nucleic acid molecules;
- comprising the steps of;
(i) providing at least one nucleic acid mixture comprising at least one first nucleic acid molecule interacting with at least one second nucleic acid molecule;
(ii) performing a ligation reaction to ligate the ends of the nucleic acid molecules to form at least one linear composite nucleic acid molecule;
and(iii) isolating at least one linear composite nucleic acid molecule comprising at least one first tag from at least one first nucleic acid molecule and at least one second tag from at least one second nucleic acid molecule;
wherein the first second tags are from different nucleic acid molecules.
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Abstract
The present invention provides at least one isolated linear composite nucleic acid molecule comprising at least one first tag from at least one first nucleic acid molecule and at least one second tag from at least one second nucleic acid molecule, wherein the first and second nucleic acids interact in a nucleic acid mixture; and wherein the first and second tags are from different nucleic acid molecules. The invention also provides a method of producing at least one isolated linear composite nucleic acid and to a method of detecting and/or identifying nucleic acid interactions.
30 Citations
25 Claims
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1. A method of preparing at least one isolated linear composite nucleic acid molecule comprising at least one first tag from at least one first nucleic acid molecule and at least one second tag from at least one second nucleic acid molecule, wherein the first and second nucleic acid molecules interact in a nucleic acid mixture and wherein the first and second tags are from different nucleic acid molecules;
- comprising the steps of;
(i) providing at least one nucleic acid mixture comprising at least one first nucleic acid molecule interacting with at least one second nucleic acid molecule; (ii) performing a ligation reaction to ligate the ends of the nucleic acid molecules to form at least one linear composite nucleic acid molecule; and (iii) isolating at least one linear composite nucleic acid molecule comprising at least one first tag from at least one first nucleic acid molecule and at least one second tag from at least one second nucleic acid molecule;
wherein the first second tags are from different nucleic acid molecules. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
- comprising the steps of;
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20. An isolated linear composite nucleic acid molecule comprising at least one first tag from at least one first nucleic acid molecule and at least one second tag from at least one second nucleic acid molecule, wherein the first and the second nucleic acid molecules interact in a nucleic acid mixture;
- and wherein the first and second tags are from different nucleic acid molecules.
- View Dependent Claims (21, 22, 23, 24, 25)
Specification