METHODS AND NUCLEIC ACIDS FOR THE ANALYSIS OF GENE EXPRESSION ASSOCIATED WITH THE PROGNOSIS OF PROSTATE CELL PROLIFERATIVE DISORDERS
First Claim
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1. A method for providing a prognosis of a subject with a prostate cell proliferative disorder comprising:
- obtaining a biological sample from a subject;
determining the expression status of at least one gene or genomic sequence selected from the group consisting PITX2, HIST2H2BF, SEQ ID NO;
63, GPR7, SEQ ID NO;
35 and FOXL2 in said sample; and
determining therefrom the prognosis of said subject whereby expression is indicative of prognosis of a prostate cell proliferative disorder.
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Abstract
Particular aspects provide novel methods and compositions (e.g., nucleic acids, kits, etc.) having substantial utility for providing a prognosis of prostate cell proliferative disorders. In particular aspects, this is achieved by the analysis of the expression status of a panel of genes, or subsets thereof.
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Citations
31 Claims
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1. A method for providing a prognosis of a subject with a prostate cell proliferative disorder comprising:
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obtaining a biological sample from a subject; determining the expression status of at least one gene or genomic sequence selected from the group consisting PITX2, HIST2H2BF, SEQ ID NO;
63, GPR7, SEQ ID NO;
35 and FOXL2 in said sample; anddetermining therefrom the prognosis of said subject whereby expression is indicative of prognosis of a prostate cell proliferative disorder. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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19. A treated nucleic acid derived from SEQ ID NOS:
- 961, 35, 63 and 19, wherein the treatment is suitable to convert at least one unmethylated cytosine base of the genomic DNA sequence to uracil or another base that is detectably dissimilar to cytosine in terms of hybridization.
- View Dependent Claims (21, 22)
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20. A nucleic acid, comprising at least 16 contiguous nucleotides of a treated genomic DNA sequence selected from the group consisting of SEQ ID NOS:
- 133, 134, 261, 262, 189, 190, 317, 318, 101, 102, 229, 230, 962-965, and sequences complementary thereto, wherein said nucleic acid is not identical or complementary to SEQ ID NOS;
961, 35, 63 and 19, wherein the treatment is suitable to convert at least one unmethylated cytosine base of the genomic DNA sequence to uracil or another base that is detectably dissimilar to cytosine in terms of hybridization.
- 133, 134, 261, 262, 189, 190, 317, 318, 101, 102, 229, 230, 962-965, and sequences complementary thereto, wherein said nucleic acid is not identical or complementary to SEQ ID NOS;
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23. An oligomer, comprising a sequence of at least 9 contiguous nucleotides that is complementary to, or hybridizes under moderately stringent or stringent conditions to a treated genomic DNA sequence selected from the group consisting of SEQ ID NOS:
- 133, 134, 261, 262, 189, 190, 317, 318, 101, 102, 229, 230, 962-965, and sequences complementary thereto, wherein said nucleic acid is not identical or complementary to SEQ ID NOS;
961, 35, 63 and 19. - View Dependent Claims (24)
- 133, 134, 261, 262, 189, 190, 317, 318, 101, 102, 229, 230, 962-965, and sequences complementary thereto, wherein said nucleic acid is not identical or complementary to SEQ ID NOS;
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25. A kit for use in for use in providing a prognosis of a subject with a prostate cell proliferative disorder, comprising:
- a means for detecting the polypeptides of a gene or genomic region selected from the group consisting of PITX2, HIST2H2BF, SEQ ID NO;
63, GPR7, FOXL2 and SEQ ID NO;
35. - View Dependent Claims (26)
- a means for detecting the polypeptides of a gene or genomic region selected from the group consisting of PITX2, HIST2H2BF, SEQ ID NO;
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27. A kit for use in providing a prognosis of a subject with a prostate cell proliferative disorder, comprising:
- a means for measuring the level of mRNA transcription of a gene or genomic region selected from the group consisting of PITX2, HIST2H2BF, SEQ ID NO;
63, GPR7, FOXL2 and SEQ ID NO;
35. - View Dependent Claims (28)
- a means for measuring the level of mRNA transcription of a gene or genomic region selected from the group consisting of PITX2, HIST2H2BF, SEQ ID NO;
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29. A kit comprising:
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at least one of a bisulfite reagent; and at least two nucleic acid molecules comprising, in each case a contiguous sequence at least 16 nucleotides that is complementary to, or hybridizes under moderately stringent or stringent conditions to a sequence selected from the group consisting of SEQ ID NOS;
133, 134, 261, 262, 189, 190, 317, 318, 101, 102, 229, 230, 962-965 and complements thereof.
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30. A composition comprising the following:
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a nucleic acid comprising a sequence at least 18 bases in length of a segment of the chemically pretreated genomic DNA according to one of the sequences taken from the group consisting of SEQ ID NOS;
133, 134, 261, 262, 189, 190, 317, 318, 101, 102, 229, 230, 962-965 and sequences complementary thereto, anda buffer comprising at least one of the following substances;
magnesium chloride, dNTP, of taq polymerase, an oligomer, in particular an oligonucleotide or peptide nucleic acid (PNA)-oligomer, said oligomer comprising in each case at least one base sequence having a length of at least 9 contiguous nucleotides which is complementary to, or hybridizes under moderately stringent or stringent conditions to a pre-treated genomic DNA according to one of the SEQ ID NOS;
133, 134, 261, 262, 189, 190, 317, 318, 101, 102, 229, 230, 962-965 and sequences complementary thereto.
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31. (canceled)
Specification