BIOLOGICAL SAMPLE REACTION CHIP AND BIOLOGICAL SAMPLE REACTION METHOD

• US 20090197274A1
• Filed: 01/27/2009
• Published: 08/06/2009
• Est. Priority Date: 02/01/2008
• Status: Active Grant

• Current Assignee: Seiko Epson Corporation (Seiko Group)
• Original Assignee: Seiko Epson Corporation (Seiko Group)
• Inventors: TAKAGI, Fumio
• Granted Patent: US 8,092,999 B2
• Time in Patent Office: Days
• US Class Current: 435/6.11
• CPC Class Codes: B01L 2200/0605 Metering of fluids
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2200/0642 Filling fluids into wells by specific techniques
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/14 Means for pressure control
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046

Abstract

A biological sample reaction chip, including: a plurality of reaction containers; a reaction liquid introduction channel having a reaction liquid supply opening at a first end and an evacuation opening at a second end; and a reaction liquid quantifying channel, a third end of which is connected to one of the reaction containers, and a fourth end of which is connected to the reaction liquid introduction channel, wherein an interior of each of the reaction containers is coated with a reagent that is necessary for a reaction.

Claims

1. A biological sample reaction chip, comprising:

a plurality of reaction containers;

a reaction liquid introduction channel having a reaction liquid supply opening at a first end and an evacuation opening at a second end; and

a reaction liquid quantifying channel, a third end of which is connected to one of the reaction containers, and a fourth end of which is connected to the reaction liquid introduction channel,

an interior of each of the reaction containers being coated with a reagent that is necessary for a reaction.

2. The biological sample reaction chip according to claim 1, wherein a volume of each of the reaction containers is smaller than a volume of the reaction liquid quantifying channels.

3. A biological sample reaction method using the biological sample reaction chips according to claim 1,

the method comprising:

reducing the pressure inside the reaction containers, the reaction liquid quantifying channels and the reaction liquid introduction channel to a predetermined pressure;

filling a reaction liquid into the reaction liquid introduction channel via the reaction liquid supply opening;

introducing the reaction liquid into the reaction liquid quantifying channels by reverting the pressure inside the reaction containers, the reaction liquid quantifying channels and the reaction liquid introduction channel to a pressure outside the chip;

removing the reaction liquid from the reaction liquid introduction channel;

introducing into the reaction containers the reaction liquid in the reaction liquid quantifying channels, by centrifugal force; and

carrying out a biological sample reaction process.

4. The biological sample reaction method according to claim 3, wherein in the reduction of the pressure to the predetermined pressure, the pressure is reduced to a pressure ranging from 50% of the pressure outside the chip to less than the pressure outside the chip.

5. The biological sample reaction method according to claim 3, wherein

the biological sample reaction process is a process including nucleic acid amplification,

the reaction liquid has a target nucleic acid, an enzyme for amplifying nucleic acid and nucleotides, at predetermined concentrations, and

the reaction containers are coated beforehand with primers.