METHODS OF CULTURING EMBRYONIC STEM CELLS AND CONTROLLED DIFFERENTIATION
First Claim
1. A method of culturing undifferentiated ES cells, said method including:
- obtaining a source of undifferentiated ES cells; and
culturing the ES cells in the presence of an indirect or direct antagonist of a BMP-mediated default pathway of extraembryonic endoderm differentiation.
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Abstract
The present invention provides a preparation of undifferentiated embryonic stem (ES) cells sustainable for a prolonged period in an undifferentiated state which will undergo stem cell renewal or somatic differentiation. Preferably the cells are capable of somatic differentiation in vitro and are inclined to differentiate away from an extraembryonic lineage. The present invention also provides method of culturing embryonic stem (ES) cells to improve stem cell maintenance and persistence in culture. The method also provides a culture of ES cells prepared by the method as well as differentiated cells derived from the embryonic cells resulting from directed differentiation procedures provided by the present invention.
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Citations
16 Claims
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1. A method of culturing undifferentiated ES cells, said method including:
- obtaining a source of undifferentiated ES cells; and
culturing the ES cells in the presence of an indirect or direct antagonist of a BMP-mediated default pathway of extraembryonic endoderm differentiation. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
- obtaining a source of undifferentiated ES cells; and
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2. A method of producing a progenitor cell from an ES cell, said method comprising:
- obtaining a source of undifferentiated ES cells; and
culturing the ES cells in the presence of an antagonist of a BMP mediated default pathway of extra embryonic endoderm differentiation for a period sufficient to differentiate the ES cell to a progenitor cell.
- obtaining a source of undifferentiated ES cells; and
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15. A method of producing a somatic cell from an undifferentiated ES cell, said method including:
- obtaining a progenitor cell comprising obtaining a source of undifferentiated ES cells;
culturing the ES cells in the presence of an antagonist of a BMP mediated default pathway of extra embryonic endoderm differentiation for a period sufficient to differentiate the ES cell to a progenitor cell;
culturing the progenitor cell in a progenitor culture medium; and
obtaining a somatic cell from a lineage of the progenitor. - View Dependent Claims (16)
- obtaining a progenitor cell comprising obtaining a source of undifferentiated ES cells;
Specification