Method and apparatus for freezing of a biological material

• US 20090202978A1
• Filed: 02/13/2008
• Published: 08/13/2009
• Est. Priority Date: 02/13/2008
• Status: Abandoned Application

• Current Assignee: Core Dynamics Limited
• Original Assignee: Core Dynamics Limited
• Inventors: Shaham, Ginadi
• Application Number:
  US12/068,953
• Publication Number:
  US 20090202978A1
• Time in Patent Office: Days
• US Class Current: 435/1.300
• CPC Class Codes: A01N 1/02 Preservation of living parts
• CPC Class Codes: A01N 1/0242 Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
• CPC Class Codes: A01N 1/0257 Stationary or portable vessels generating cryogenic temperatures
• CPC Class Codes: A01N 1/0268 Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen
• CPC Class Codes: C12N 1/04 Preserving or maintaining viable microorganisms immobilised microorganisms C12N11/00
• CPC Class Codes: F25D 3/10 using liquefied gases, e.g. liquid air for cooling semiconductor devices H01L23/445
• CPC Class Codes: Y10T 29/49 Method of mechanical manufacture

Abstract

The present invention provides a device for freezing biological material, comprising: a cooling unit; a heat insulation unit, having an interface with said cooling unit and a common longitudinal axis passing through said interface transversely thereto; an elongated slot disposed at least in said heat insulation unit and extending along said axis, said slot comprising a first slot portion remote from said interface and a second slot portion adjacent said interface; wherein said first and second slot portions have respective first and second dimensions measured perpendicularly to said slot'"'"'s axis, the former being greater than the latter.

Further provided is a container for accommodating therein of a biological material, said container having a longitudinal container axis and comprising a first container portion having a first dimension and a second container portion having a second dimension smaller than the first dimension, both measured perpendicularly to said longitudinal container axis; the container portions being in fluid communication; said container being adapted for carrying said biological material in said first container portion, and a solution different from said material, fully filling said second container portion, which is essentially free of said biological material, and at least partially filling said first container portion so as to be in contact with said biological material; said container further comprising retention means for preventing passage of said biological material from said first container portion to said second container portion.

Methods for freezing biological material making use of the device and container of the invention is also part of the invention.

Claims

1. A device for freezing biological material, comprising:

a cooling unit;

a heat insulation unit, having an interface with said cooling unit and a common longitudinal axis passing through said interface transversely thereto;

an elongated slot disposed at least in said heat insulation unit and extending along said axis, said slot comprising a first slot portion remote from said interface and a second slot portion adjacent said interface;

wherein said first and second slot portions have respective first and second dimensions measured perpendicularly to said slot'"'"'s axis, the former being greater than the latter.

2. A device according to claim 1, wherein said slot is adapted for receiving therein a container having a first container portion for accommodating said biological material and a second container portion free of said material, such that when the container is mounted within said slot, said first container portion is disposed in the first slot portion and a portion of the second container portion is within said second slot portion.

3. A device according to claim 1, wherein the cooling unit comprises a chamber for holding a cooling fluid medium.

4. A device according to claim 3, wherein the heat insulation unit is fixed to said chamber.

5. A device according to claim 3, wherein said fluid medium is a liquid and said heat insulation unit floats on top of said liquid.

6. A device according to claim 1, wherein the cooling unit is in a form of a solid cooling block, said block comprising a recess which abuts said interface and is in fluid communication with said elongated slot disposed in said heat insulation unit.

7. A device according to claim 2, wherein the cooling unit is in a form of a solid cooling block, said block comprising a recess which abuts said interface and is in fluid communication with said elongated slot disposed in said heat insulation unit wherein said recess is adapted for having thermoconductive contact with said second container portion.

8. A device according to claim 6, wherein said recess is adapted for accommodating a cooling liquid therein.

9. A container for accommodating therein of a biological material, said container having a longitudinal container axis and comprising a first container portion having a first dimension and a second container portion having a second dimension smaller than the first dimension, both measured perpendicularly to said longitudinal container axis;

the container portions being in fluid communication;

said container being adapted for carrying said biological material in said first container portion, and a solution different from said material, fully filling said second container portion, which is essentially free of said biological material, and at least partially filling said first container portion so as to be in contact with said biological material;

said container further comprising retention means for preventing passage of said biological material from said first container portion to said second container portion.

10. A container according to claim 9, adapted to be open at least at one end thereof.

11. A container according to claim 9, wherein said retention means are attached to a stopper adapted to be received within an opening of said first container portion.

12. A container according to claim 9, wherein said retention means are in the form of a mesh located within said first container portion.

13. A container according to claim 9, wherein said retention means is a membrane.

14. A container according to claim 9, wherein said retention means comprises a cup-like segment extending from said first portion at an opening of the container being remote from said second container portion, the cup-like segment having a dimension measured perpendicularly to the container'"'"'s axis being greater than the dimension of said first portion, both measured perpendicularly to said axis.

15. A container according to claim 9, comprising a protecting pouch or a sleeve made of a sheet of sealable material wrapped around and attached to the circumference of a portion thereof forming an extension of said container.

16. A container according to claim 15, wherein said container is placed within said protecting pouch.

17. A kit for freezing biological material, comprising:

(a) a device comprising;

a cooling unit;

a heat insulation unit, having an interface with said cooling unit and a common longitudinal axis passing through said interface transversely thereto;

an elongated slot disposed at least in said heat insulation unit and extending along said axis, said slot comprising a first slot portion remote from said interface and a second slot portion adjacent said interface;

wherein said first and second slot portions have respective first and second dimensions measured perpendicularly to said axis, the former being greater than the latter; and

(b) a container for accommodating therein of said biological material, said container having a longitudinal container axis and comprising a first container portion having a first dimension and a second container portion having a second dimension smaller than the first dimension, both measured perpendicularly to said container axis;

the container portions being in fluid communication;

said container being adapted for carrying said biological material in said first container portion, and a solution different from said material, fully filling said second container portion, which is free of said biological material, at least partially filling said first container portion so as to be in contact with said biological material;

said container further comprising retention means for preventing passage of said biological material from said first container portion to said second container portion.

18. A kit according to claim 17, further comprising a protective pouch for placing said container therein.

19. A method for freezing biological material comprising:

providing a container having a longitudinal container axis and comprising a first container portion having a first dimension and a second container portion having a second dimension smaller than the first dimension, both measured perpendicularly to said container axis;

the container portions being in fluid communication;

introducing into said container a solution and a biological material, such that said second container portion is free of said biological material and is fully filled with said solution and said first container portion is at least partially filled with said solution; and

cooling a part of said second container portion which is remote from said first container portion with a cooling means, thereby cooling the solution therein, while insulating said first container portion and a remainder of said second container portion.