MSMB-gene based diagnosis, staging and prognosis of prostate cancer
First Claim
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1. A method of identifying a prostate cell proliferative disorder in a human male subject, the method comprising:
- providing a sample comprising prostatic tissue, prostatic cells, fluid of the prostate from a human patient susceptible to a prostate cancer, andanalyzing the sample for the level DNA methylation of the regulatory region surrounding the transcription start site (TSS) of a beta-microseminoprotein gene (MSMB gene), wherein hypermethylation in this region indicates the presence of prostate cancer cells or is indicative of prostate cancer.
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Abstract
This invention relates generally to a method of diagnosis for distinguishing between a benign prostate hyperplasia and a prostate cancer and between a hormone-sensitive and a hormone-refractory prostate cancer condition and specifically to identification of a hypermethylated (on CpG and non-CpG dinucleotides) CpG island in the beta-microseminoprotein (MSMB) regulatory regions surrounding the transcriptional start site of the MSMB gene as a diagnostic indicator of prostate cancer (PrCa) and for distinguishing androgen-refractory from androgen-sensitive prostate cancer.
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41 Claims
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1. A method of identifying a prostate cell proliferative disorder in a human male subject, the method comprising:
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providing a sample comprising prostatic tissue, prostatic cells, fluid of the prostate from a human patient susceptible to a prostate cancer, and analyzing the sample for the level DNA methylation of the regulatory region surrounding the transcription start site (TSS) of a beta-microseminoprotein gene (MSMB gene), wherein hypermethylation in this region indicates the presence of prostate cancer cells or is indicative of prostate cancer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40)
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41. A nucleic acid molecule consisting essentially of a sequence at least 16 continuous bases in length of a sequence selected from the sequence group consisting of SEQ ID NOS:
- 6 and 8.
Specification