ANALYSIS

• US 20090203022A1
• Filed: 02/09/2009
• Published: 08/13/2009
• Est. Priority Date: 02/07/2008
• Status: Abandoned Application

• Current Assignee: Forensic Science Service Limited (Government of United Kingdom), Whitespace Enterprise Corporation
• Original Assignee: Forensic Science Service Limited (Government of United Kingdom), Arizona Board of Regents (University of Arizona)
• Inventors: Nordquist, Alan, ZENHAUSERN, Frederic, Tully, Gill, Koumi, Pieris, Lenigk, Ralf, Hurth, Cedric, Hopwood, Andrew
• Application Number:
  US12/368,031
• Publication Number:
  US 20090203022A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/6848 characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]
• CPC Class Codes: C12Q 2545/113 with an external standard/control, i.e. control reaction is separated from the test/target reaction

Abstract

A method of analysing a sample includes providing a first part of the sample and a second part of the sample. A first analysis is conducted on the first part of the sample and the results of the first analysis are considered. A second analysis is conducted on the second part of the sample, the second analysis being conducted according to a procedure using a value for each of one or more characteristics of the procedure. The consideration of the results of the first analysis is used to determine whether the value for one or more of the characteristics of the procedure is changed to a different value. The second analysis is started before the results of the first analysis are obtained.

Claims

1. A method of analysing a sample, the method including:

providing a first part of the sample;

providing a second part of the sample;

conducting a first analysis on the first part of the sample;

considering the results of the first analysis;

conducting a second analysis on the second part of the sample, the second analysis being conducted according to a procedure, the procedure using a value for each of one or more characteristics of the procedure;

wherein the consideration of the results of the first analysis is used to determine whether the value for one or more of the characteristics of the procedure is changed to a different value; and

wherein the second analysis is started before the results of the first analysis are obtained.

2. The method of claim 1, wherein the first analysis and the second analysis start within 5 minutes of the other analysis starting.

3. The method of claim 1 wherein the first analysis takes less than 60 minutes to complete.

4. The method of claim 1 wherein the first analysis is completed at least 30 minutes before the scheduled completion of the second analysis.

5. The method of claim 1 wherein the first part and the second part have the same volume ±

1%.

6. The method of claim 1 wherein the ratio of sample to reagents is the same in respect of the first part and in respect of the second part ±

1%.

7. The method of claim 1 wherein the first analysis provides information on one or more characteristics of the first part of the sample, the one or more characteristics being one or more of:

the quantity of nucleic acid;

the quantity of amplifiable nucleic acid;

the quantity of amplifiable nucleic acid of a given type;

the presence of one or more inhibitors to amplification;

the extent of degradation of the nucleic acid;

the presence of Y chromosome nucleic acid;

the quantity of Y chromosome nucleic acid;

the presence of nucleic acid from two or more different sources;

the ratio of the nucleic acid from one source to the nucleic acid from another source.

8. The method of claim 1 wherein the second analysis is conducted according to a procedure which has a standard form and the standard form of the procedure is used in the second analysis unless the consideration of the results from the first analysis suggest a change to the procedure.

9. The method of claim 1 wherein the procedure is defined in terms of one or more characteristics, and the value for a characteristic is a number of PCR cycles or a PCR cycle operating temperature or a length of time for a PCR cycle.

10. The method of claim 1 wherein the value for one or more of the characteristics of the procedure is changed in response to the first analysis indicating the presence of one or more inhibitors in the sample.

11. The method of claim 1 wherein the quantity of nucleic acid is detected in the first analysis and is compared with a reference quantity or reference range of quantities, the reference quantity or range of quantities being associated with one or more values to use in the second analysis, where the quantity detected in the first analysis is below the reference quantity or reference range of quantities, the number of cycles being increased compared with the value of cycles associated with the references and/or where the quantity detected in the first analysis is above the reference quantity or reference range of quantities, the number of cycles being decreased compared with the value of cycles associated with the references.

12. The method of claim 1 wherein the consideration of the results of the first analysis is used to determine whether the value for one or more of the characteristics of the procedure is changed to a different value and the value is in respect of a value used in the second analysis in the physical processing of the second part of the sample or in the second analysis in the second analysis data processing of the results for the second part of the sample.

13. The method of claim 1 wherein the results of the first analysis indicate whether or not the sample is from a mixture of sources and/or the ratio of nucleic acid from one source to that from another source in the mixture.

14. A method of analysing a sample, the method including:

processing in parallel a first analysis method and a second analysis method, the results of the first analysis method being considered and used to amend the second analysis method before it is completed.

15. Apparatus for analysing a sample, the including:

a sample introduction location;

a first chamber in fluid communication with the sample introduction location;

a first chamber process controller;

a first detector for one or more properties of a first part of a sample processed in the first chamber according to the first chamber process controller;

a first analysis data processor receiving signals from the first detector and providing signals to a second process controller;

a second chamber in fluid communication with the sample introduction location, the second chamber processing a second part of the sample according to the second process controller;

a second detector for one or more properties of the second part of the sample processed in the second chamber;

the second process controller providing a procedure for application to the second chamber, the procedure using a value for each of one or more characteristics of the procedure, the procedure being provided for use when the signals received from the first analysis data processor are of a given form, the second process controller providing a further procedure for application to the second chamber, the further procedure using a different value for one or more of the characteristics, the further procedure being applied to the second chamber in response to the signals provided by the first data processor to the second process controller when the signals differ from the given form, and wherein the signals from the first analysis data processor are provided to the second process controller after the second process controller has started applying the procedure to the second chamber.

16. Apparatus for analysing a sample, the including:

a sample introduction location;

a first chamber in fluid communication with the sample introduction location;

a first chamber process controller;

a first detector for one or more properties of a first part of a sample processed in the first chamber according to the first chamber process controller;

a first analysis data processor receiving signals from the first detector and providing signals to a second analysis data processor;

a second chamber in fluid communication with the sample introduction location;

a second chamber process controller;

a second detector for one or more properties of the second part of the sample processed in the second chamber according to the second chamber process controller;

the second analysis data processor providing a procedure for application to signals received from the second detector, the procedure using a value for each of one or more characteristics of the procedure, the procedure being provided for use when the signals received from the first analysis data processor are of a given form, the second analysis data processor providing a further procedure for application to the signals from the second detector, the further procedure using a different value for one or more of the characteristics, the further procedure being applied to the signals from the second detector to the second process controller when the signals differ from the given form, and wherein the signals from the first analysis data processor are provided to the second analysis data processor after the second process controller has started applying the process to the second chamber.