PROCESS FOR PRODUCING POLYPEPTIDE
First Claim
1. A process for producing a polypeptide which comprises culturing a rat cell line adapted to a medium which does not contain serum (hereinafter referred to as a serum-free medium) in a serum-free medium, and recovering the desired polypeptide from the culture.
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Accused Products
Abstract
The present invention relates to a process for producing a desired polypeptide using rat cells. Specifically, the present invention relates to a process for producing the polypeptide which comprises culturing rat cells such as YB2/3HL.P2.G11.16Ag.20 (hereinafter referred to as YB2/0), preferably rat cells to which a recombinant DNA comprising DNA encoding a desired polypeptide such as an immunologically functional molecule is introduced, in a medium which does not contain serum (hereinafter referred to as a serum-free medium). Among the desired polypeptides obtained by the process of the present invention, an antibody obtained by using a transformant of YB2/0 has a high antibody-dependent cell-mediated cytotoxic activity (hereinafter sometimes referred to as ADCC activity) and is useful as a pharmaceutical agent.
23 Citations
24 Claims
- 1. A process for producing a polypeptide which comprises culturing a rat cell line adapted to a medium which does not contain serum (hereinafter referred to as a serum-free medium) in a serum-free medium, and recovering the desired polypeptide from the culture.
-
17. A process for adapting a rat cell to a serum-free medium, which comprises inoculating rat cells into a conditioned medium at a cell density of 1×
- 105 to 1×
106 cells/ml. - View Dependent Claims (18, 19, 20, 21, 22, 23)
- 105 to 1×
-
24. A rat cell line adapted to a serum-free medium, 61-33γ
- (FERM BP-7325).
Specification