ZYMOMONAS WITH IMPROVED ETHANOL PRODUCTION IN MEDIUM CONTAINING CONCENTRATED SUGARS AND ACETATE
First Claim
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1. A process for producing ethanol from a mixed-sugars medium comprising xylose, comprising:
- (a) providing a recombinant Zymomonas strain capable of utilizing xylose to produce ethanol, said strain comprising at least one genetic modification which reduces expression of integration host factor alpha subunit protein (HimA); and
(b) culturing the strain of (a) in a medium comprising xylose, whereby xylose is used by said strain as a carbon source for producing ethanol.
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Abstract
Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas strains further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in mixed-sugars medium comprising xylose, and, in particular, in the presence of acetate.
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Citations
12 Claims
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1. A process for producing ethanol from a mixed-sugars medium comprising xylose, comprising:
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(a) providing a recombinant Zymomonas strain capable of utilizing xylose to produce ethanol, said strain comprising at least one genetic modification which reduces expression of integration host factor alpha subunit protein (HimA); and (b) culturing the strain of (a) in a medium comprising xylose, whereby xylose is used by said strain as a carbon source for producing ethanol. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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Specification