Method of classifying antibody, method of identifying antigen, method of obtaining antibody or antibody set, method of constructing antibody panel and antibody or antibody set and use of the same
First Claim
1. A method of classifying antibody including the following steps:
- (1) preparing a plurality of antibodies recognizing cell surface antigen;
(2) bringing each of the antibodies into contact with cells of the same kinds;
(3) analyzing each cell after step (2) by flow cytometry so as to obtain data showing reactivity between the antibody and the cell surface; and
(4) comparing the obtained data and classifying antibodies based on the similarity of the data.
3 Assignments
0 Petitions
Accused Products
Abstract
It is intended to provide a method whereby a plural number of antibodies against cell surface antigens are quickly classified and to provide a method whereby antigens of the thus classified antibodies are quickly identified. Further, it is intended to provide a method of promoting the utilization of the useful data obtained by the above methods. Furthermore, it is intended to provide an antibody which is effective in treating or diagnosing cancer. Namely, a method of classifying antibodies which comprises: (1) the step of preparing a plural number of antibodies respectively recognizing cell surface antigens; (2) the step of bringing each of these antibodies into contact with a cell of the same species; (3) the step of analyzing each of the cells having been treated in the step (2) by flow cytometry and thus obtaining data indicating the reactivity of each antibody with its cell surface antigen; and (4) the step of comparing the thus obtained data and classifying the individual antibodies depending on the similarity. A method of identifying antigens which further comprises: (5) the step of selecting one to several antibodies from each antibody group formed in the step (4) and identifying antigens thereof; and (6) on the assumption that antigens of the antibodies belonging to a single antibody group are the same or highly related to one another, making relations between the antigens having been identified in the step (5) and the antibody groups to thereby identify the antigens. An antibody against HER1, an antibody against HER2, an antibody against CD46, an antibody against ITGA3, an antibody against ICAM1, an antibody against ALCAM, an antibody against CD147, an antibody against C1qR, an antibody against CD44, an antibody against CD73, an antibody against EpCAM and an antibody against HGFR, each obtained by using the above methods.
50 Citations
63 Claims
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1. A method of classifying antibody including the following steps:
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(1) preparing a plurality of antibodies recognizing cell surface antigen; (2) bringing each of the antibodies into contact with cells of the same kinds; (3) analyzing each cell after step (2) by flow cytometry so as to obtain data showing reactivity between the antibody and the cell surface; and (4) comparing the obtained data and classifying antibodies based on the similarity of the data. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 55, 56, 57, 58, 59)
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26. An identifying method of an antigen including the following steps:
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(1) preparing a plurality of antibodies recognizing cell surface antigen; (2) bringing each of the antibodies into contact with cells of the same kind; (3) analyzing each cell after step (2) by flow cytometry so as to obtain data showing the reactivity between the antibody and the cell surface; (4) comparing the obtained data and classifying antibodies based on the similarity of the data; (5) selecting one or several antibodies from each antibody group formed in the step (4) and identifying an antigen thereof; and (6) associating the antigens identified in the step (5) with an antibody group, based on the estimation that antigens to antibodies belonging to the same antibody group are identical or have high relationship. - View Dependent Claims (27, 28, 29, 30, 31, 32)
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54. (canceled)
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60. A method of selecting an optimum treatment method of a certain disease, the method comprising the following steps:
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(1) preparing a panel displaying a reactivity between one or more antibodies selected from the group consisting of 048-006 antibody, 015-126 antibody, 067-133 antibody, 064-044 antibody, 076-048 antibody and 059-053 antibody, and a clinical cancer tissue of one or more diseases selected from the group consisting of squamous carcinoma, adenosquamous carcinoma, alveolar adenocarcinoma, adenocarcinoma, and large cell carcinoma, and a cell or tissue separated from a subject; (2) examining a reactivity between the cell or the tissue and each antibody displayed on the panel; (3) collating the results in the step (2) with the panel, and (4) selecting an effective antibody according to the results of collating. - View Dependent Claims (61, 62)
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63-85. -85. (canceled)
Specification