Specific binding sites in collagen for integrins and use thereof
First Claim
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1. An expression vector, comprising:
- a DNA sequence encoding a recombinant collagen or collagen-like protein of SEQ ID NO;
15 or SEQ ID NO;
21;
wherein amino acid Xaa in both sequences is hydroxyproline.
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Abstract
The present invention identified a high affinity binding sequence in collagen type III for the collagen-binding integrin I domains. Provided herein are the methods used to characterize the sequence, the peptides comprising this novel sequence and the use of the peptides in enabling cell adhesion. Also provided herein are methods to identify specific integrin inhibitors, sequences of these inhibitors and their use in inhibiting pathophysiological conditions that may arise due to integrin-collagen interaction.
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Citations
18 Claims
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1. An expression vector, comprising:
a DNA sequence encoding a recombinant collagen or collagen-like protein of SEQ ID NO;
15 or SEQ ID NO;
21;
wherein amino acid Xaa in both sequences is hydroxyproline.- View Dependent Claims (2)
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3. A method of identifying an inhibitor of an integrin-collagen interaction, comprising:
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designing a test compound comprising a sequence that is specifically recognized by the I domain of the integrin, wherein said design is based on computer-aided molecular modeling; and comparing the level of binding of the integrin to the collagen in the presence or absence of the test compound, wherein a decrease in binding of the integrin to the collagen in presence of the test compound indicates that the test compound is an inhibitor of the integrin-collagen interaction. - View Dependent Claims (4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification