SENSORS EMPLOYING COMBINATORIAL ARTIFICIAL RECEPTORS
First Claim
1. A detector system comprising:
- a competitor comprising an analog of a ligand of interest covalently coupled to a macromolecule;
an artificial receptor configured to bind a ligand of interest and the competitor, binding of the ligand of interest competing with binding of the competitor;
the artificial receptor comprising a plurality of different building block molecules independently covalently coupled to a solid support in a region;
the region being a contiguous portion of the surface of the solid support with the different building block molecules distributed randomly throughout the contiguous region;
a semipermeable membrane that is permeable to the ligand of interest but that retains an effective amount of the competitor;
the semipermeable membrane and the artificial receptor at least partially defining a chamber, the competitor and the artificial receptor being in the chamber; and
a detector operatively coupled to the artificial receptor and configured to, in response to binding of the competitor or the ligand of interest to the artificial receptor, produce indicia of presence or concentration of the ligand of interest.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention relates to sensors including artificial receptors and methods of using them. In an embodiment, the present invention includes an artificial receptor as a component of a receptor system including a ligand permeable interface that isolates the artificial receptor from certain components of the surrounding environment. In an embodiment, the present invention includes an artificial receptor and a competitor against a ligand of interest.
In an embodiment, the present invention includes a competitive artificial receptor as a component of a detector system including a semipermeable membrane that isolates the competitive artificial receptor from certain components of the surrounding environment. This embodiment also includes the competitor and a detector operatively coupled to the competitive artificial receptor. The detector produces a signal indicating binding of the competitor and/or the ligand of interest to the artificial receptor. The detector system is configured so that the competitor is retained in the environs of the artificial receptor at least in part by the ligand permeable interface.
20 Citations
30 Claims
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1. A detector system comprising:
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a competitor comprising an analog of a ligand of interest covalently coupled to a macromolecule; an artificial receptor configured to bind a ligand of interest and the competitor, binding of the ligand of interest competing with binding of the competitor; the artificial receptor comprising a plurality of different building block molecules independently covalently coupled to a solid support in a region;
the region being a contiguous portion of the surface of the solid support with the different building block molecules distributed randomly throughout the contiguous region;a semipermeable membrane that is permeable to the ligand of interest but that retains an effective amount of the competitor; the semipermeable membrane and the artificial receptor at least partially defining a chamber, the competitor and the artificial receptor being in the chamber; and a detector operatively coupled to the artificial receptor and configured to, in response to binding of the competitor or the ligand of interest to the artificial receptor, produce indicia of presence or concentration of the ligand of interest. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method of determining a level of a ligand of interest, comprising:
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implanting in a subject a detector system; retrieving from the detector system indicia of presence or concentration of the ligand of interest; the detector system comprising; a competitor comprising an analog of a ligand of interest covalently coupled to a macromolecule; an artificial receptor configured to bind a ligand of interest and the competitor, binding of the ligand of interest competing with binding of the competitor; the artificial receptor comprising a plurality of different building block molecules independently covalently coupled to a solid support in a region;
the region being a contiguous portion of the surface of the solid support with the different building block molecules distributed randomly throughout the contiguous region;a semipermeable membrane that is permeable to the ligand of interest but that retains an effective amount of the competitor; the semipermeable membrane and the artificial receptor at least partially defining a chamber, the competitor and the artificial receptor being in the chamber; and a detector operatively coupled to the artificial receptor and configured to, in response to binding of the competitor or the ligand of interest to the artificial receptor, produce indicia of presence or concentration of the ligand of interest. - View Dependent Claims (11, 12, 13, 14, 15, 16)
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17. An artificial receptor system comprising:
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a molecular competitor that competes against binding of a ligand of interest; an artificial receptor configured to bind a ligand of interest and the competitor, binding of the ligand of interest competing with binding of the competitor;
the artificial receptor comprising a plurality of different building block molecules independently covalently coupled to a solid support;a ligand permeable interface that is permeable to the ligand of interest but that retains an effective amount of the competitor;
the ligand permeable interface and the artificial receptor at least partially defining a chamber, the competitor and the artificial receptor being in the chamber; anda detector operatively coupled to the artificial receptor and configured to produce indicia of presence or concentration of the ligand of interest. - View Dependent Claims (18, 19, 20, 21)
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22. A method of determining a level of a ligand of interest, comprising:
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implanting in a subject a detector system; retrieving from the detector system indicia of presence or concentration of the ligand of interest; the detector system comprising; a molecular competitor that competes against binding of a ligand of interest; an artificial receptor configured to bind a ligand of interest and the competitor, binding of the ligand of interest competing with binding of the competitor;
the artificial receptor comprising a plurality of different building block molecules independently covalently coupled to a solid support;a ligand permeable interface that is permeable to the ligand of interest but that retains an effective amount of the competitor;
the ligand permeable interface and the artificial receptor at least partially defining a chamber, the competitor and the artificial receptor being in the chamber; anda detector operatively coupled to the artificial receptor and configured to produce indicia of presence or concentration of the ligand of interest. - View Dependent Claims (23, 24, 25, 26, 27, 28)
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29. An artificial receptor system comprising:
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a competitor comprising an analog of a ligand of interest covalently coupled to a macromolecule; an artificial receptor configured to bind a ligand of interest and the competitor, binding of the ligand of interest competing with binding of the competitor; the artificial receptor comprising a plurality of different building block molecules independently covalently coupled to a solid support in a region;
the region being a contiguous portion of the surface of the solid support with the different building block molecules distributed randomly throughout the contiguous region.
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30. An artificial receptor system comprising:
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a molecular competitor that competes against binding of a ligand of interest; an artificial receptor configured to bind a ligand of interest and the competitor, binding of the ligand of interest competing with binding of the competitor;
the artificial receptor comprising a plurality of different building block molecules independently covalently coupled to a solid support.
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Specification