HIGH-DENSITY ION TRANSPORT MEASUREMENT BIOCHIP DEVICES AND METHODS
First Claim
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10. An ion channel chip comprising:
- a. a planar substrate;
b. at least one aperture positioned substantially perpendicular to said planar substrate;
c. a lipid positioned about said aperture, wherein a cell may continuously contact a portion of said lipid and said aperture.
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Abstract
The present invention includes biochips for the measurement of cellular ion channels and methods of use and manufacture. The biochips of the present invention have enhanced sealing capabilities provided in part by chemically modifying the surface of the biochip surface or substrate or by exposure to an ionized gas. The present invention also includes novel cartridges for biochips.
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Citations
27 Claims
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10. An ion channel chip comprising:
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a. a planar substrate; b. at least one aperture positioned substantially perpendicular to said planar substrate; c. a lipid positioned about said aperture, wherein a cell may continuously contact a portion of said lipid and said aperture. - View Dependent Claims (1, 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 13, 14, 15, 16, 17, 19, 26)
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16-1. The method according to claim 15, wherein said modifying the surface comprises exposing said chip substrate to an acid or a base.
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18. An ion channel chip comprising:
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a. a planar polymer plastic substrate; b. a glass surface layer positioned on opposing sides of said planar polymer plastic substrate; and c. at least one aperture positioned perpendicular to said glass surface layers and said polymer plastic substrate.
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20. A method of laser drilling an ion channel chip comprising:
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a. providing a chip substrate;
wherein regions of said chip substrate not to be laser drilled are maskedb. providing a laser; c. splitting the beam from said laser into two or more beamlets; and d. focusing said beamlets through at least one lens onto said chip substrate thereby creating a throughbore or counterbore. - View Dependent Claims (21, 22)
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23. A method of manufacturing a substrate for an ion channel chip comprising:
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a. treating a first glass surface with an acid; b. treating a second glass surface with a base; c. contacting said first glass surface with said second glass surface; d. heating said surfaces to a temperature capable of bonding said first glass surface to said second glass surface.
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24. A method of manufacturing a substrate for an ion channel chip comprising:
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a. treating a first glass surface with a base; b. treating a second glass surface with a base; c. applying Na2SiO3 to said first glass surface; d. contacting said second glass surface to said applied Na2SiO3; and e. heading said surfaces to a temperature capable of bonding said Na2SiO3 to said glass surfaces.
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25. A vacuum gasket for use with an ion channel chip comprising:
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a. a planar surface having a length and width approximately equal to the length and width of at least one ion channel chip; b. a sealing structure protruding parallel to said planar surface, wherein said sealing structure is capable of encircling the perimeter of an aperture of said ion channel chip when engaged; c. a vacuum groove permitting the removal of air from between said planar surface and said chip thereby creating a vacuum seal; and wherein said sealing structure defines a chamber when said gasket is sealed to said ion channel chip.
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27. A storage structure for an ion channel chip comprising:
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a. a top structure; b. a bottom structure comprising a series of chip engaging structures, wherein each engaging structure is capable of engaging an ion channel chip;
further wherein said bottom structure reversible engages said top structure.
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Specification