ANALYTE SENSORS AND METHODS OF USE

• US 20090211344A1
• Filed: 05/08/2009
• Published: 08/27/2009
• Est. Priority Date: 12/22/2006
• Status: Active Grant

• Current Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories Incorporated)
• Original Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories Incorporated)
• Inventors: Wang, Yi
• Granted Patent: US 8,033,162 B2
• Time in Patent Office: Days
• US Class Current: 73/61.410
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0825 Test strips
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 27/416 Systems G01N27/27 takes precedence
• CPC Class Codes: Y10T 156/1082 Partial cutting bonded sandwich [e.g., grooving or incising]

Abstract

Analyte sensors for determining the concentration of an analyte in a sample. The sensors have a sample chamber having an inlet with a projection extending from an edge of the sensor for facilitating flow of sample into the sample chamber.

Claims

1-24. -24. (canceled)

25. An sensor for determining the concentration of a ketone body in a sample, the sensor comprising a sample chamber having an inlet with a width and a projection extending from an edge of the sensor proximate the inlet, the projection having a width that is less than the inlet width.

26. The sensor of claim 25, wherein the width is no more than about 80% of the inlet width.

27. The sensor of claim 25, wherein the width is about 50% of the inlet width.

28. The sensor of claim 25, wherein the projection has a height of at least about 0.2 mm.

29. The sensor of claim 25, wherein the sensor comprises a first substrate and a second substrate, a spacer between the first substrate and the second substrate, together the first substrate, second substrate and spacer defining the inlet, and wherein the projection is defined by at least one of the first substrate and the second substrate.

30. The sensor of claim 29, wherein the projection extends from a side edge of the substrate.

31. The sensor of claim 29, wherein the projection extends from an end edge of the substrate.

32. The sensor of claim 29, wherein the projection is defined by the first substrate and the sensor further comprises a second projection defined by the second substrate.

33. The sensor of claim 25, wherein the sample chamber has a volume of no more than about 1 microliter.

34. A sensor for determining the concentration of a ketone body in a sample, the sensor comprising:

a first substrate, a second substrate, and a spacer layer therebetween;

a sample chamber defined between the first substrate and the second substrate bounded by the spacer layer, the sample chamber having at least one inlet;

a protrusion extending from the first substrate at the inlet.

35. The sensor of claim 34, further comprising a second protrusion extending from the second substrate at the inlet.

36. The sensor of claim 34, further comprising a second inlet to the sample chamber and a third protraction extending from the first substrate at the second inlet.

37. The sensor of claim 36, further comprising a fourth protrusion extending from the second substrate at the second inlet.

38. A sensor for determining the concentration of a ketone body in a sample, the sensor comprising:

a first substrate and a second substrate;

a first side edge, a second side edge and an end edge;

a sample chamber defined between the first substrate and the second substrate, the sample chamber extending from the first side edge to the second side edge;

a first aperture and a second aperture between the first substrate and the second substrate at the first side edge and the second side edge, respectively; and

at least one protrusion extending from either the first side edge or the second side edge, proximate the apertures.

39. The sensor of claim 38, comprising a first protrusion extending from the first side edge and a second protrusion extending from the second side edge.

40. The sensor of claim 39, further comprising a third projection extending from the first side edge and a fourth projection extending from the second side edge, proximate the apertures.

41. The sensor of claim 40, wherein the width of each of the projections is less than the width of the proximate aperture.

42. The sensor of claim 40, wherein the width of each of the projections is no more than about 80% of the width of the proximate aperture.

43. The sensor of claim 40, wherein the projections have a height of at least about 0.2 mm.

44. The sensor of claim 40, wherein the projections are triangles extending from the substrates.

45. A method of analyzing a ketone body concentration in a sample comprising:

contacting a sample with a sensor;

transferring the sample into a sample chamber through an inlet bounded by at least one protrusion that has a width less than a width of the inlet; and

determining the concentration of the ketone body in the sample.

46. The method of claim 45, wherein transferring comprises transferring the sample into the sample chamber through an inlet bounded by two protrusion that have a width less than the width of the inlet.

47. A method of analyzing a ketone body concentration in a sample comprising:

contacting a sample with a sensor having a first substrate, a second substrate and a spacer layer therebetween and at least one protrusion extending from one of the substrates;

transferring the sample into a sample chamber through an inlet bounded by the substrates and the at least one protrusion; and

determining the concentration of the ketone body in the sample.

48. The method of claim 47, wherein transferring comprises transferring the sample into the sample chamber through an inlet bounded by two protrusions.