Layered peptide/antigen arrays - for high-throughput antibody screening of clinical samples
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Accused Products
Abstract
A method and composition for the identification of biomolecule in a sample are disclosed. The method comprises obtaining a coated capture membrane stack comprising a plurality of capture membranes with each capture membrane coated with a different peptide. The membrane stack is exposed to a sample, and, after a given amount of time for the sample to permeate the membrane stack, the membrane stack is removed from the sample carrier and the capture membrane to which the biomolecule adheres is identified.
9 Citations
26 Claims
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17. The method of claim 17, wherein said toxic events are selected from the group of bacterial toxins and environmental toxins.
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21. A method for identifying a histopathological tissue sample, said method comprising:
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a) obtaining said histopathological sample; b) exposing said histopathological sample to conjugate antibodies, said conjugate antibodies comprising; i) a primary antibody, said primary antibody having specificity for certain specific peptides of a sample; ii) a shuttle antibody attached to said primary antibody, forming a bispecific antibody conjugate; c) obtaining a coated capture membrane stack, said membrane stack comprising a plurality of coated capture membranes, each said coated capture membrane of said coated capture membrane stack comprising; i) a membrane; and ii) a peptide coating said membrane, wherein each said peptide coating of each said coated capture membrane differs from other said peptide coatings of said coated capture membranes of said coated capture membrane stack; d) washing away any unreacted antibodies, e) exposing said coated capture membrane stack to said histophathological sample for a given time period to allow for cleaving of conjugated antibodies at the site of the primary and the shuttle antibody conjugation, allowing for said shuttle antibodies to migrate to any said peptide coated membrane for which said shuttle antibody is specific; f) separating each said coated capture membrane from said membrane stack; and g) identifying said histological sample, by identifying at least each said coated capture membrane to which at least one said shuttle antibody adheres. - View Dependent Claims (14, 16, 23)
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22. A kit for identifying antibodies, said kit comprising:
a membrane stack, said membrane stack comprising; a) a plurality of capture membranes, each said capture membrane being a track etched membrane and; b) each said capture membrane coated with a different peptide.
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24. A method for identifying antigens of a histopathological tissue sample, said method comprising:
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a) obtaining said histopathological sample; b) exposing said histopathological sample to antibodies which target specific antigens on a histopathological sample; c) obtaining a coated capture membrane stack, said membrane stack comprising a plurality of coated capture membranes, each said coated capture membrane of said coated capture membrane stack comprising; i) a membrane; and ii) a peptide coating said membrane, wherein each said peptide coating of each said coated capture membrane differs from other said peptide coatings of said coated capture membranes of said coated capture membrane stack; d) exposing said coated capture membrane stack to said histopathological sample for a given time period to allow said antibodies to migrate to any said peptide coated membrane for which said antibodies are specific; and f) identifying said antigen of said histological sample, by identifying at least each said coated capture membrane to which said antibodies adhere. - View Dependent Claims (2, 25, 26)
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Specification