Proteomic Fingerprinting of Human IVF-Derived Embryos: Identification of Biomarkers of Developmental Potential
First Claim
1. A method for aiding in a diagnosis of developmental potential in an IVF-derived human embryo comprising:
- a) culturing said IVF-derived human embryo in embryo culture media;
b) obtaining a test sample from said embryo culture media;
c) detecting the quantity of at least one biomarker in said test sample, said at least one biomarker selected from the group consisting of biomarkers having a molecular weight of about 2454±
5, 2648±
5.3, 2665±
5.3, 2684±
5.4, 2778±
5.6, 4093±
8.2, 37820±
76, 45873±
92, 282390±
565 and 435170±
870 Daltons;
d) comparing the quantity of said at least one biomarker in said test sample to the quantity of the same said at least one biomarker in a control sample known to lack favorable developmental potential;
e) wherein the differential expression of said at least one biomarker in said test sample relative to said control sample is correlated with a diagnosis of favorable developmental potential.
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Abstract
The present invention discloses biomarkers and biomarker combinations that have prognostic value as predictors of the developmental potential of individual IVF-derived human embryos. In particular, the biomarkers of this invention are useful to classify an embryo with implantation competence after uterine transfer or implantation incompetence. In addition, the biomarkers can be detected by non-invasive methods that do not harm the developing embryo. Also disclosed are kits for the prediction of developmental potential that detect the biomarkers of the invention, as well as methods using a plurality of classifiers to make a probable diagnosis of developmental potential.
21 Citations
39 Claims
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1. A method for aiding in a diagnosis of developmental potential in an IVF-derived human embryo comprising:
-
a) culturing said IVF-derived human embryo in embryo culture media; b) obtaining a test sample from said embryo culture media; c) detecting the quantity of at least one biomarker in said test sample, said at least one biomarker selected from the group consisting of biomarkers having a molecular weight of about 2454±
5, 2648±
5.3, 2665±
5.3, 2684±
5.4, 2778±
5.6, 4093±
8.2, 37820±
76, 45873±
92, 282390±
565 and 435170±
870 Daltons;d) comparing the quantity of said at least one biomarker in said test sample to the quantity of the same said at least one biomarker in a control sample known to lack favorable developmental potential; e) wherein the differential expression of said at least one biomarker in said test sample relative to said control sample is correlated with a diagnosis of favorable developmental potential. - View Dependent Claims (5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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2. A method for aiding in a diagnosis of developmental potential in an IVF-derived human embryo comprising:
-
a) culturing said IVF-derived human embryo in embryo culture media; b) obtaining a test sample from said embryo culture media; c) detecting the quantity of at least one biomarker in said test sample, said at least one biomarker selected from the group consisting of biomarkers having a molecular weight of about 2454±
5, 2648±
5.3, 2665±
5.3, 2684±
5.4, 2778±
5.6 and 4093±
8.2 Daltons;d) comparing the quantity of said at least one biomarker in said test sample to the quantity of the same said at least one biomarker in a control sample known to lack favorable developmental potential; e) wherein the underexpression of said at least one biomarker in said test sample relative to said control sample is correlated with a diagnosis of favorable developmental potential.
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3. A method for aiding in a diagnosis of developmental potential in an IVF-derived human embryo comprising:
-
a) culturing said IVF-derived human embryo in embryo culture media; b) obtaining a test sample from said embryo culture media; c) detecting the quantity of at least one biomarker in said test sample, said at least one biomarker selected from the group consisting of biomarkers having a molecular weight of about 2454±
5, 2648±
5.3, 2665±
5.3, 2684±
5.4 and 2778±
5.6 Daltons;d) comparing the quantity of said at least one biomarker in said test sample to the quantity of the same said at least one biomarker in a control sample known to lack favorable developmental potential; e) wherein the underexpression of said at least one biomarker in said test sample relative to said control sample is correlated with a diagnosis of favorable developmental potential.
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4. A method for aiding in a diagnosis of developmental potential in an IVF-derived human embryo comprising:
-
a) culturing said IVF-derived human embryo in embryo culture media; b) obtaining a test sample from said embryo culture media; c) detecting at least one biomarker in said test sample, said at least one biomarker selected from the group consisting of biomarkers having a molecular weight of about 37820±
76, 45873±
92, 282390±
565 and 435170±
870 Daltons;d) wherein the detection of said at least one biomarker in said test sample is correlated with a diagnosis of favorable developmental potential.
-
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25. A kit for aiding in a diagnosis of developmental potential in an IVF-derived human embryo comprising one or more container means comprising an adsorbent comprising at least one capture reagent attached thereto, wherein the capture reagent binds at least one biomarker;
- wherein said at least one biomarker is selected from the group consisting of biomarkers having a molecular weight of about 2454±
5, 2648±
5.3, 2665±
5.3, 2684±
5.4, 2778±
5.6, 4093±
8.2, 7820±
76, 45873±
92, 282390±
565 and 435170±
870 Daltons. - View Dependent Claims (28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39)
- wherein said at least one biomarker is selected from the group consisting of biomarkers having a molecular weight of about 2454±
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26. A kit for aiding in a diagnosis of developmental potential in an IVF-derived human embryo comprising one or more container means comprising an adsorbent comprising at least one capture reagent attached thereto, wherein the capture reagent binds at least one biomarker;
- wherein said at least one biomarker is selected from the group consisting of biomarkers having a molecular weight of about 2454±
5, 2648±
5.3, 2665±
5.3, 2684±
5.4, 2778±
5.6 Daltons.
- wherein said at least one biomarker is selected from the group consisting of biomarkers having a molecular weight of about 2454±
-
27. A kit for aiding in a diagnosis of developmental potential in an IVF-derived human embryo comprising one or more container means comprising an adsorbent comprising at least one capture reagent attached thereto, wherein the capture reagent binds at least one biomarker;
- wherein said at least one biomarker is selected from the group consisting of biomarkers having a molecular weight of about 7820±
76, 45873±
92, 282390±
565 and 435170±
870 Daltons.
- wherein said at least one biomarker is selected from the group consisting of biomarkers having a molecular weight of about 7820±
Specification